Human SUMO-conjugating enzyme UBC9 ELISA Kit | UBE2I elisa kit

Human SUMO-conjugating enzyme UBC9 ELISA Kit

Cat. Num. AAA761609

• Gene Names: UBE2I; P18; UBC9; C358B7.1
• Reactivity: Human
• Synonyms: SUMO-conjugating enzyme UBC9; Human SUMO-conjugating enzyme UBC9 ELISA Kit; UBE2I/SUMO-conjugating enzyme UBC9/Ubiquitin-conjugating Enzyme E2I/p18/Ubiquitin-protein ligase I/Ubiquitin-conjugating enzyme E2 I/Ubiquitin carrier protein I/SUMO-protein ligase/Ubiquitin carrier protein 9/UBC9/UBCE9/C358B7.1; UBE2I elisa kit

• Reactivity: Human
• Sequence Length: 158

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for UBE2I elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human UBE2I antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human UBE2I antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human UBE2I amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human UBE2I can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 35494003
• NCBI GeneID: 7329
• NCBI Accession #: NP_919237.1
• NCBI GenBank Nucleotide #: NM_194261.2
• Molecular Weight: 18,007 Da
• NCBI Official Full Name: SUMO-conjugating enzyme UBC9
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2I
• NCBI Official Symbol: UBE2I
• NCBI Official Synonym Symbols: P18; UBC9; C358B7.1
• NCBI Protein Information: SUMO-conjugating enzyme UBC9
• UniProt Protein Name: SUMO-conjugating enzyme UBC9
• Protein Family: SUMO-conjugating enzyme
• UniProt Gene Name: UBE2I
• UniProt Synonym Gene Names: UBC9; UBCE9
• UniProt Entry Name: UBC9_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

UBC9: Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly- SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Interacts with HIPK1, HIPK2, PPM1J, RASD2 and TCF3 Interacts with NR2C1; the interaction promotes its sumoylation. Forms a tight complex with RANGAP1 and RANBP2. Interacts with SIAH1 and PARP. Interacts with various transcription factors such as TFAP2A, TFAP2B, TFAP2C, AR, ETS1 and SOX4. Interacts with RWDD3; the interaction enhances the sumoylation of a number of proteins such as HIF1A and I-kappa-B. Interacts with DNMT1. Interacts with FOXL2. Forms a complex with SENP6 and UBE2I in response to UV irradiation. Interacts with human herpesvirus 6 IE2. Interacts with human adenovirus early E1A protein; this interaction interferes with polysumoylation (Probable). Interacts with DNM1l (via its GTPase and B domains); the interaction promotes sumoylation of DNM1L, mainly in its B domain. Interacts with PML-RARA oncoprotein (via the coiled-colied domain); the interaction is required for sumoylation of the PML- RARA oncoprotein. Interacts with IPO13. Interacts with NFATC2IP; this inhibits formation of poly-SUMO chains. Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: EC 6.3.2.19; Ligase; SUMO conjugating system; Nuclear receptor co-regulator; Ubiquitin conjugating system; EC 6.3.2.-; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cytoplasm; cytosol; nuclear envelope; nucleoplasm; nucleus; PML body; synaptonemal complex

Molecular Function: ATP binding; enzyme binding; HLH domain binding; ligase activity; protein binding; SUMO ligase activity; transcription factor binding; ubiquitin protein ligase binding

Biological Process: cell division; cellular protein metabolic process; chromosome segregation; DNA repair; double-strand break repair; double-strand break repair via homologous recombination; mitosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nucleotide-excision repair; positive regulation of I-kappaB kinase/NF-kappaB cascade; post-translational protein modification; protein modification process; protein sumoylation; ubiquitin-dependent protein catabolic process; viral reproduction