Rabbit anti-Human UBE2E2 Polyclonal Antibody | anti-UBE2E2 antibody

UBE2E2 Antibody

Cat. Num. AAA9417027

• Gene Names: UBE2E2; UBCH8
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: UBE2E2; Polyclonal Antibody; UBE2E2 Antibody; UBCH8; anti-UBE2E2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total UBE2E2 protein.
• Purity/Purification: Antigen affinity purification
• Form/Format: Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Concentration: 0.6mg/ml (varies by lot)
• Sequence Length: 201

• Applicable Applications for anti-UBE2E2 antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1: 20-100
• Immunogen: Full length fusion protein
• Immunogen Type: Protein
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using UBE2E2 Antibody at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200))

Related Product Information for anti-UBE2E2 antibody

Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). The first step in the ubiquitination process requires the ATP-dependent activation of the ubiquitin C-terminus and the assembly of multi-ubiquitin chains by the E1 enzyme. The ubiquitin chain is then conjugated to the E2 enzyme to generate an intermediate ubiquitin-E2 complex. The E3 enzyme then catalyzes the transfer of ubiquitin from E2 to the appropriate protein substrate, thereby targeting that substrate for degradation. A wide range of enzymes facilitate this proteolytic ubiquitin pathway, one of which is UBE2E2 (also known as UBCH8 in human), which functions as an E2 enzyme and catalyzes the ATP-dependent covalent attachment of ubiquitin to target proteins, thereby playing an important role in protein degradation.

Product Categories/Family for anti-UBE2E2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 22749327
• NCBI GeneID: 7325
• NCBI Accession #: NP_689866.1
• NCBI GenBank Nucleotide #: NM_152653.3
• UniProt Accession #: Q96LR5
• Molecular Weight: 22,255 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 E2
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 E2
• NCBI Official Symbol: UBE2E2
• NCBI Official Synonym Symbols: UBCH8
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 E2
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 E2
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2E2
• UniProt Synonym Gene Names: UBCH8
• UniProt Entry Name: UB2E2_HUMAN

Uniprot Description

UBE2E2: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 3p24.2

Molecular Function: ISG15 conjugating enzyme activity; protein binding; ubiquitin-protein ligase activity

Biological Process: ISG15-protein conjugation; response to DNA damage stimulus