Testing Data
(Gel: 8%SDS-PAGE Lysates (from left to right): Hela, Jurkat, 293T and K562 cell, mouse testis tissue Amount of lysate: 40ug per lane Primary antibody: 1/550 dilution Secondary antibody dilution: 1/8000 Exposure time: 40 seconds)
Rabbit anti-Human, Mouse ARIH2 Polyclonal Antibody | anti-ARIH2 antibody
ARIH2 Antibody
Gene Names
ARIH2; ARI2; TRIAD1
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Antigen affinity purification.
Synonyms
ARIH2; Polyclonal Antibody; ARIH2 Antibody; ARI2; TRIAD1; anti-ARIH2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total ARIH2 protein.
Purity/Purification
Antigen affinity purification.
Form/Format
Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Concentration
2.2 mg/ml (varies by lot)
Sequence Length
493
Applicable Applications for anti-ARIH2 antibody
Western Blot (WB)
Application Notes
Western blotting: 1:500-1:2000
Immunogen Type
Peptide
Immunogen Description
Synthetic peptide corresponding to a region derived from internal residues of human ariadne homolog 2 (Drosophila)
Target Name
ARIH2
Preparation and Storage
Store at -20 degree C
Testing Data
(Gel: 8%SDS-PAGE Lysates (from left to right): Hela, Jurkat, 293T and K562 cell, mouse testis tissue Amount of lysate: 40ug per lane Primary antibody: 1/550 dilution Secondary antibody dilution: 1/8000 Exposure time: 40 seconds)
Testing Data
(Gel: 8%SDS-PAGE Lysates (from left to right): Hela, Jurkat, 293T and K562 cell, mouse testis tissue Amount of lysate: 40ug per lane Primary antibody: 1/550 dilution Secondary antibody dilution: 1/8000 Exposure time: 40 seconds)
Related Product Information for anti-ARIH2 antibody
TRIAD1, also known as ARIH2 (ariadne homolog 2) or ARI2, is a 493 amino acid protein that contains one IBR-type zinc finger and two RING-type zinc fingers and belongs to the ariadne subfamily of RBR proteins. Localized to the nucleus, TRIAD1 interacts with UBE2L3 and is thought to act as an E3 ubiquitin-protein ligase, functioning to accept ubiquitin from E2 ubiquitin-conjugating enzymes and transfer the acquired ubiquitin residue to target substrates. TRIAD1 is subject to post-translational DNA damage-dependent phosphorylation, probably by ATM or ATR. The gene encoding TRIAD1 maps to human chromosome 3, which houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci.
Product Categories/Family for anti-ARIH2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,819 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase ARIH2
NCBI Official Synonym Full Names
ariadne RBR E3 ubiquitin protein ligase 2
NCBI Official Symbol
ARIH2
NCBI Official Synonym Symbols
ARI2; TRIAD1
NCBI Protein Information
E3 ubiquitin-protein ligase ARIH2
UniProt Protein Name
E3 ubiquitin-protein ligase ARIH2
Protein Family
UniProt Gene Name
ARIH2
UniProt Synonym Gene Names
ARI2; TRIAD1; ARI-2; Protein ariadne-2 homolog
UniProt Entry Name
ARI2_HUMAN
Uniprot Description
ARIH2: E3 ubiquitin-protein ligase mediating 'Lys-48'-and 'Lys- 63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. May play a role in myelopoiesis. Belongs to the RBR family. Ariadne subfamily.
Protein type: Ligase; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.-
Chromosomal Location of Human Ortholog: 3p21
Cellular Component: cytoplasm; nucleus
Molecular Function: protein binding; nucleic acid binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity
Biological Process: developmental cell growth; protein polyubiquitination; multicellular organismal development; protein ubiquitination during ubiquitin-dependent protein catabolic process
Research Articles on ARIH2
Similar Products
Product Notes
The ARIH2 arih2 (Catalog #AAA9408532) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARIH2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ARIH2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting: 1:500-1:2000. Researchers should empirically determine the suitability of the ARIH2 arih2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARIH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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