Rabbit anti-Human UBE2D4 Polyclonal Antibody | anti-UBE2D4 antibody

Anti-UBE2D4 Antibody, Rabbit Polyclonal

Cat. Num. AAA8106837

• Gene Names: UBE2D4; HBUCE1
• Reactivity: Human
• Applications: ELISA
• Purity: Protein A
• Synonyms: UBE2D4; Polyclonal Antibody; Anti-UBE2D4 Antibody; Rabbit Polyclonal; UBE2D4 Antibody; Rabbit PAb; ubiquitin-conjugating enzyme E2D 4 (putative); Anti-HBUCE1 Antibody; anti-UBE2D4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human UBE2D4
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-UBE2D4 antibody: ELISA (EIA)
• Application Notes: ELISA: 1:1000-1:2000
• Immunogen: Recombinant Human UBE2D4 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human UBE2D4 (rh UBE2D4). Total IgG was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-UBE2D4 antibody

UBE2D4 is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by a ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin-protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans. It has been identified the UBE2D family (UBE2D1-4) as E2 partners for IDOL that support both autoubiquitination and IDOL-dependent ubiquitination of the LDLR in a cell-free system.

References

Sjoerd J L van Wijk, et al. (2009) A comprehensive framework of E2-RING E3 interactions of the human ubiquitin-proteasome system. Mol Syst Biol. 5: 317.Zhang L, et al. (2011) The IDOL-UBE2D complex mediates sterol-dependent degradation of the LDL receptor. Genes Dev. 25 (12): 1262-74.Nandi D, et al. (2006) The ubiquitin-proteasome system. Journal of biosciences. 31 (1): 137-55.

NCBI and Uniprot Product Information

• NCBI GI #: 8393719
• NCBI GeneID: 51619
• NCBI Accession #: NP_057067.1
• NCBI GenBank Nucleotide #: NM_015983.3
• UniProt Accession #: Q9Y2X8; A4D1V0
• Molecular Weight: 16,649 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 D4
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2D 4 (putative)
• NCBI Official Symbol: UBE2D4
• NCBI Official Synonym Symbols: HBUCE1
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 D4; ubiquitin carrier protein D4; ubiquitin-conjugating enzyme HBUCE1; ubiquitin-protein ligase D4
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 D4
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2D4
• UniProt Synonym Gene Names: UBCH5D
• UniProt Entry Name: UB2D4_HUMAN

Uniprot Description

UBE2D4: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Ligase; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 7p13

Molecular Function: protein binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: protein ubiquitination