Rabbit anti-Human UBE1L Polyclonal Antibody | anti-UBE1L antibody

UBE1L Antibody

Cat. Num. AAA9504778

• Gene Names: UBA7; D8; UBE2; UBA1B; UBE1L
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Synonyms: UBE1L; Polyclonal Antibody; UBE1L Antibody; anti-UBE1L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: UBE1L Antibody detects endogenous levels of total UBE1L protein.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1,012

• Applicable Applications for anti-UBE1L antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:10000
• Target Modification: Unmodified/Total
• Immunogen: The antiserum was produced against synthesized peptide derived from human UBE1L.
• Immunogen Range: 963-1012
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using UBE1L Antibody. The picture on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of lysates from HeLa cells, using UBE1L Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 38045948
• NCBI GeneID: 7318
• NCBI Accession #: NP_003326.2
• NCBI GenBank Nucleotide #: NM_003335.2
• UniProt Accession #: P41226; Q9BRB2
• Molecular Weight: 111 kDa
• NCBI Official Full Name: ubiquitin-like modifier-activating enzyme 7
• NCBI Official Synonym Full Names: ubiquitin like modifier activating enzyme 7
• NCBI Official Symbol: UBA7
• NCBI Official Synonym Symbols: D8; UBE2; UBA1B; UBE1L
• NCBI Protein Information: ubiquitin-like modifier-activating enzyme 7
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme 7
• UniProt Gene Name: UBA7
• UniProt Synonym Gene Names: UBE1L; UBE2; Ubiquitin-activating enzyme 7

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein. [provided by RefSeq, Jul 2008]

Uniprot Description

Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Catalyzes the ISGylation of influenza A virus NS1 protein.