Testing Data
(Human growth hormone detected with Mouse anti Human growth hormone followed by HRP conjugated Rabbit F(ab')2 anti Mouse IgG antibody .)
Mouse anti-Human GROWTH HORMONE Monoclonal Antibody
MOUSE ANTI HUMAN GROWTH HORMONE
Reactivity
Human
Applications
ELISA
Synonyms
GROWTH HORMONE; Monoclonal Antibody; MOUSE ANTI HUMAN GROWTH HORMONE; Growth Hormone antibody|5B4; anti-GROWTH HORMONE antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
5B4
Specificity
GROWTH HORMONE
Form/Format
Purified IgG, liquid. Phosphate buffered saline
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Applicable Applications for anti-GROWTH HORMONE antibody
ELISA (EIA)
Application Notes
ELISA: 1/5000-1/50000
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Species Cross-Reactivity
Bovine, Pig, Horse
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(Human growth hormone detected with Mouse anti Human growth hormone followed by HRP conjugated Rabbit F(ab')2 anti Mouse IgG antibody .)
Testing Data
(Human growth hormone detected with Mouse anti Human growth hormone followed by HRP conjugated Rabbit F(ab')2 anti Mouse IgG antibody .)
Related Product Information for anti-GROWTH HORMONE antibody
Mouse anti hGH, Clone 5B4, recognises human growth hormone, also known as somatotropin, a polypeptide synthesised by acidophilic or somatotropic cells of the anterior pituitary gland. It exists as several isoforms, including a ~20 kDa and ~22 kDa variant. Growth hormone plays an important role in growth and cell production.
References
1. Gomez, F. et al. (1984) A highly sensitive radioimmunoassay for human growth hormone using a monoclonal antibody.J Immunoassay. 5:145-57.
2. Hennen, G. et al. (1985) A human placental GH: increasing levels during second half of pregnancy with pituitary GH suppression as revealed by monoclonal antibody radioimmunoassays.Int J Fertil. 30: 27-33.
3. Frankenne, F. et al. (1987) [Discovery of a placental variant of human growth hormone: biochemistry, physiology and implication in the secretion of hypophyseal forms].Reprod Nutr Dev. 27: 523-4.
4. Frankenne, F. et al. (1988) The physiology of growth hormones (GHs) in pregnant women and partial characterization of the placental GH variant.J Clin Endocrinol Metab. 66: 1171-80.
5. Igout, A. et al. (1989) Expression and secretion of the human placental growth hormone in Escherichia coli.Nucleic Acids Res. 17: 3998.
2. Hennen, G. et al. (1985) A human placental GH: increasing levels during second half of pregnancy with pituitary GH suppression as revealed by monoclonal antibody radioimmunoassays.Int J Fertil. 30: 27-33.
3. Frankenne, F. et al. (1987) [Discovery of a placental variant of human growth hormone: biochemistry, physiology and implication in the secretion of hypophyseal forms].Reprod Nutr Dev. 27: 523-4.
4. Frankenne, F. et al. (1988) The physiology of growth hormones (GHs) in pregnant women and partial characterization of the placental GH variant.J Clin Endocrinol Metab. 66: 1171-80.
5. Igout, A. et al. (1989) Expression and secretion of the human placental growth hormone in Escherichia coli.Nucleic Acids Res. 17: 3998.
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Product Notes
The GROWTH HORMONE (Catalog #AAA225995) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN GROWTH HORMONE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GROWTH HORMONE can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). ELISA: 1/5000-1/50000 Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the GROWTH HORMONE for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GROWTH HORMONE, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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