Rabbit UBAP1 Polyclonal Antibody | anti-UBAP1 antibody

UBAP1 Antibody

Cat. Num. AAA9603292

• Gene Names: UBAP1; UAP; UBAP; NAG20; UBAP-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: UBAP1; Polyclonal Antibody; UBAP1 Antibody; MGC8710; NAG20; Nasopharyngeal carcinoma associated gene 20 protein; UAP; UBAP 1; UBAP; Ubiquitin associated protein 1; anti-UBAP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: UBAP1 antibody detects endogenous levels of UBAP1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 566

• Applicable Applications for anti-UBAP1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human UBAP1.
• Subcellular Location: Cytoplasm; Cytosol. Endosome. Note: Predominantly cytosolic. Recruited to endosomes as part of the ESCRT-I complex.
• Tissue Specificity: Ubiquitous. Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle and pancreas.
• Predicted Cross Reactivity: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
• Similarity: Pig (100%), Zebrafish (83%), Bovine (92%), Horse (100%), Sheep (100%), Rabbit (92%), Chicken (92%), Xenopus (92%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9603292 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of extracts of A431 cells, using UBAP1 antibody.)

Related Product Information for anti-UBAP1 antibody

Function: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Plays a role in the proteasomal degradation of ubiquitinated cell-surface proteins, such as EGFR and BST2.
Subunit Structure: Component of an ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, VPS37A and UBAP1 in a 1:1:1:1 stoichiometry. Can be a component of ESCRT-I complexes containing VPS37B, VPS37C and VPS37D (in vitro) (PubMed:22405001), but may have a preference for the ESCRT-I complex containing VPS37A (PubMed:21757351). Interacts with PTPN23. Interacts (via UBA domains) with ubiquitinated proteins.
Similarity: The UMA domain mediates association with the ESCRT-I complex.

NCBI and Uniprot Product Information

• NCBI GI #: 283945558
• NCBI GeneID: 51271
• NCBI Accession #: NP_001164672.1
• NCBI GenBank Nucleotide #: NM_001171201.1
• UniProt Accession #: Q9NZ09; Q4V759; Q53FP7; Q5T7B3; Q6FI75; Q8NC52; Q8NCG6; B7Z348; B7Z8N9; D3DRL7; F5GXE2; F5H0J8
• Molecular Weight: Observed: 55 kDa; Predicted: 56 kDa
• NCBI Official Full Name: ubiquitin-associated protein 1 isoform 2
• NCBI Official Synonym Full Names: ubiquitin associated protein 1
• NCBI Official Symbol: UBAP1
• NCBI Official Synonym Symbols: UAP; UBAP; NAG20; UBAP-1
• NCBI Protein Information: ubiquitin-associated protein 1
• UniProt Protein Name: Ubiquitin-associated protein 1
• Protein Family: UBAP1-MVB12-associated
• UniProt Gene Name: UBAP1
• UniProt Synonym Gene Names: UBAP-1

NCBI Description

This gene is a member of the UBA domain family, whose members include proteins having connections to ubiquitin and the ubiquitination pathway. The ubiquitin associated domain is thought to be a non-covalent ubiquitin binding domain consisting of a compact three helix bundle. This particular protein originates from a gene locus in a refined region on chromosome 9 undergoing loss of heterozygosity in nasopharyngeal carcinoma (NPC). Taking into account its cytogenetic location, this UBA domain family member is being studies as a putative target for mutation in nasopharyngeal carcinomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Uniprot Description

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Plays a role in the proteasomal degradation of ubiquitinated cell-surface proteins, such as EGFR and BST2.