Western Blot (WB)
(Western blot analysis of extracts from 293 cells using UBAC1 antibody.)
Rabbit anti-Human UBAC1 Polyclonal Antibody | anti-UBAC1 antibody
UBAC1 Antibody
Gene Names
UBAC1; KPC2; GBDR1; UBADC1
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
UBAC1; Polyclonal Antibody; UBAC1 Antibody; 1110033G07Rik; AA407978; E3 ubiquitin-protein ligase subunit KPC2; GBDR1; Glialblastoma cell differentiation-related protein 1; Glialblastoma differentiation-related protein 1; Kip1 ubiquitination-promoting complex protein 2; kpc 2; Kpc2; OTTHUMP00000022563; Putative glialblastoma cell differentiation-related protein; RP11-432J22.3; UBA domain containing 1; UBA domain-containing protein 1; UBAC 1; Ubadc1; Ubiquitin associated domain containing 1; Ubiquitin-associated domain-containing protein 1; anti-UBAC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
UBAC1 antibody detects endogenous levels of total UBAC1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
405
Applicable Applications for anti-UBAC1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasmic
Tissue Specificity
Ubiquitous.
Predicted Cross Reactivity
Zebrafish, Horse, Dog, Chicken, Xenopus
Similarity
Zebrafish (80%), Horse (80%), Dog (80%), Chicken (80%), Xenopus (90%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from 293 cells using UBAC1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells using UBAC1 antibody.)
Immunofluorescene (IF)
(MBS9606052 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescene (IF)
(MBS9606052 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-UBAC1 antibody
Function: Non-catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.
Subunit Structure: Component of the KPC complex composed of RNF123/KPC1 and UBAC1/KPC2. Interacts with RNF123 via its N-terminal domain. Interacts with the proteasome via its N-terminal domain.
Subunit Structure: Component of the KPC complex composed of RNF123/KPC1 and UBAC1/KPC2. Interacts with RNF123 via its N-terminal domain. Interacts with the proteasome via its N-terminal domain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 45 kD
Predicted: 46 kDa
Predicted: 46 kDa
NCBI Official Full Name
ubiquitin-associated domain-containing protein 1
NCBI Official Synonym Full Names
UBA domain containing 1
NCBI Official Symbol
UBAC1
NCBI Official Synonym Symbols
KPC2; GBDR1; UBADC1
NCBI Protein Information
ubiquitin-associated domain-containing protein 1
UniProt Protein Name
Ubiquitin-associated domain-containing protein 1
Protein Family
UniProt Gene Name
UBAC1
UniProt Synonym Gene Names
GBDR1; KPC2; UBADC1; UBA domain-containing protein 1
Uniprot Description
Non-catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.
Research Articles on UBAC1
Similar Products
Product Notes
The UBAC1 ubac1 (Catalog #AAA9606052) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBAC1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UBAC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the UBAC1 ubac1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBAC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
