Western Blot (WB)
(The anti-STK31 Pab is used in Western blot to detect STK31 in rat testis tissue lysate.)
Rabbit anti-Human, Rat STK31 Polyclonal Antibody | anti-STK31 antibody
STK31, NT (Serine/Threonine-protein Kinase 31, Serine/Threonine-protein Kinase NYD-SPK, SgK396, SGK396, Sugen Kinase 396) (FITC)
Gene Names
STK31; TDRD8; SGK396
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
STK31; Polyclonal Antibody; NT (Serine/Threonine-protein Kinase 31; Serine/Threonine-protein Kinase NYD-SPK; SgK396; SGK396; Sugen Kinase 396) (FITC); anti-STK31 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human STK31. Species Crossreactivity: rat.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-STK31 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human STK31 (KLH).
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(The anti-STK31 Pab is used in Western blot to detect STK31 in rat testis tissue lysate.)
Western Blot (WB)
(The anti-STK31 Pab is used in Western blot to detect STK31 in rat testis tissue lysate.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Western Blot (WB)
(STK31 Antibody (F47) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the STK31 antibody detected the STK31 protein (arrow).)
Western Blot (WB)
(STK31 Antibody (F47) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the STK31 antibody detected the STK31 protein (arrow).)
Product Categories/Family for anti-STK31 antibody
References
1. Wang, P.J., et al., Nat. Genet. 27(4):422-426 (2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~86 kDa
NCBI Official Full Name
Homo sapiens serine/threonine kinase 31 (STK31), transcript variant 1, mRNA
NCBI Official Synonym Full Names
serine/threonine kinase 31
NCBI Official Symbol
STK31
NCBI Official Synonym Symbols
TDRD8; SGK396
NCBI Protein Information
serine/threonine-protein kinase 31; sugen kinase 396; tudor domain containing 8; serine/threonine-protein kinase NYD-SPK
UniProt Protein Name
Serine/threonine-protein kinase 31
Protein Family
UniProt Gene Name
STK31
UniProt Synonym Gene Names
SGK396; SgK396
UniProt Entry Name
STK31_HUMAN
NCBI Description
This gene is similar to a mouse gene that encodes a putative protein kinase with a tudor domain, and shows testis-specific expression. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Tissue specificity: Testis specific.
Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.Contains 1 protein kinase domain.Contains 1 Tudor domain.
Caution: Ser-854 is present instead of the conserved Asp which is expected to be an active site residue.
Research Articles on STK31
Similar Products
Product Notes
The STK31 stk31 (Catalog #AAA6500186) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The STK31, NT (Serine/Threonine-protein Kinase 31, Serine/Threonine-protein Kinase NYD-SPK, SgK396, SGK396, Sugen Kinase 396) (FITC) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's STK31 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-1:100 FLISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the STK31 stk31 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STK31, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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