Rabbit anti-Human UBAC1 Polyclonal Antibody | anti-UBAC1 antibody

UBAC1, CT (UBAC1, GBDR1, KPC2, UBADC1, Ubiquitin-associated domain-containing protein 1, E3 ubiquitin-protein ligase subunit KPC2, Glialblastoma cell differentiation-related protein 1, Kip1 ubiquitination-promoting complex protein 2) (Biotin)

Cat. Num. AAA6360565

• Gene Names: UBAC1; GBDR1; UBADC1; RP11-432J22.3
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: UBAC1; Polyclonal Antibody; CT (UBAC1; GBDR1; KPC2; UBADC1; Ubiquitin-associated domain-containing protein 1; E3 ubiquitin-protein ligase subunit KPC2; Glialblastoma cell differentiation-related protein 1; Kip1 ubiquitination-promoting complex protein 2) (Biotin); Kip1 ubiquitination-promoting complex protein 2; anti-UBAC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-UBAC1 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: UBAC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272-302 amino acids from the C-terminal region of human UBAC1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(UBAC1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the UBAC1 antibody detected the UBAC1 protein (arrow).)

Immunohistochemistry (IHC)

(UBAC1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the UBAC1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(UBAC1 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-UBAC1 antibody

UBAC1 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein has two RRM domains that bind to RNAs.

Product Categories/Family for anti-UBAC1 antibody

Antibodies; Ubiquitin Ligases

References

Walker, L.C., et al. Breast Cancer Res. Treat. 112(2):229-236(2008). Wu, Y.Y., et al. Cell Biochem. Funct. 26(4):467-477(2008). Reboll, M.R., et al. RNA 13(8):1328-1340(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 55770884
• NCBI GeneID: 10422
• NCBI Accession #: NP_057256.2
• NCBI GenBank Nucleotide #: NM_016172.2
• UniProt Accession #: Q9BSL1; O75500; Q9UMW7
• Molecular Weight: 45,338 Da
• NCBI Official Full Name: ubiquitin-associated domain-containing protein 1
• NCBI Official Synonym Full Names: UBA domain containing 1
• NCBI Official Symbol: UBAC1
• NCBI Official Synonym Symbols: GBDR1; UBADC1; RP11-432J22.3
• NCBI Protein Information: ubiquitin-associated domain-containing protein 1; UBA domain-containing protein 1; E3 ubiquitin-protein ligase subunit KPC2; ubiquitin associated domain containing 1; kip1 ubiquitination-promoting complex protein 2; glialblastoma cell differentiation-rela
• UniProt Protein Name: Ubiquitin-associated domain-containing protein 1
• Protein Family: Ubiquitin-associated domain-containing protein
• UniProt Gene Name: UBAC1
• UniProt Synonym Gene Names: GBDR1; KPC2; UBADC1; UBA domain-containing protein 1
• UniProt Entry Name: UBAC1_HUMAN

Uniprot Description

UBAC1: Non-catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: Golgi apparatus; cytoplasm; plasma membrane

Molecular Function: protein binding

Biological Process: protein ubiquitination