Human Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA) ELISA Kit | UACA elisa kit

Human Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA) ELISA Kit

Cat. Num. AAA2707001

• Gene Names: UACA; NUCLING
• Reactivity: Human
• Synonyms: Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA); Human Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA) ELISA Kit; NUCLING; UACA elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA).; No significant cross-reactivity or interference between Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA) and analogues was observed.
• Sequence Length: 1,403

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.108ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for UACA elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 59850762
• NCBI GeneID: 55075
• NCBI Accession #: NP_060473.2
• NCBI GenBank Nucleotide #: NM_018003.3
• UniProt Accession #: Q9BZF9; Q14DD3; Q8N3B8; Q96NH6; Q9HCL1; Q9NWC6; G3XAG2
• Molecular Weight: 161,510 Da
• NCBI Official Full Name: uveal autoantigen with coiled-coil domains and ankyrin repeats isoform 1
• NCBI Official Synonym Full Names: uveal autoantigen with coiled-coil domains and ankyrin repeats
• NCBI Official Symbol: UACA
• NCBI Official Synonym Symbols: NUCLING
• NCBI Protein Information: uveal autoantigen with coiled-coil domains and ankyrin repeats
• UniProt Protein Name: Uveal autoantigen with coiled-coil domains and ankyrin repeats
• Protein Family: Uveal autoantigen with coiled-coil domains and ankyrin repeats
• UniProt Gene Name: UACA
• UniProt Synonym Gene Names: KIAA1561

NCBI Description

This gene encodes a protein that contains ankyrin repeats and coiled coil domains and likely plays a role in apoptosis. Studies in rodents have implicated the encoded protein in the stimulation of apoptosis and the regulation of mammary gland involution, in which the mammary gland returns to its pre-pregnant state. This protein has also been proposed to negatively regulate apoptosis based on experiments in human cell lines in which the protein was shown to interact with PRKC apoptosis WT1 regulator protein, also known as PAR-4, and inhibit translocation of the PAR-4 receptor. Autoantibodies to this protein have been identified in human patients with panuveitis and Graves' disease. Differential expression of this gene has been observed in various human cancers. [provided by RefSeq, May 2017]

Uniprot Description

Regulates APAF1 expression and plays an important role in the regulation of stress-induced apoptosis. Promotes apoptosis by regulating three pathways, apoptosome up-regulation, LGALS3/galectin-3 down-regulation and NF-kappa-B inactivation. Regulates the redistribution of APAF1 into the nucleus after proapoptotic stress. Down-regulates the expression of LGALS3 by inhibiting NFKB1 ().