Rabbit anti-Human UBA52 Polyclonal Antibody | anti-UBA52 antibody

UBA52, CT (Ubiquitin-60S Ribosomal Protein L40, CEP52, Ubiquitin A-52 Residue Ribosomal Protein Fusion Product 1, UBCEP2)

Cat. Num. AAA647638

• Gene Names: UBA52; L40; CEP52; RPL40; HUBCEP52
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: UBA52; Polyclonal Antibody; CT (Ubiquitin-60S Ribosomal Protein L40; CEP52; Ubiquitin A-52 Residue Ribosomal Protein Fusion Product 1; UBCEP2); Anti -UBA52; anti-UBA52 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human UBA52.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-UBA52 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, ELISA and Immunohistochemistry.; Dilution: ELISA: 1:50-1:100; Western Blot: 1:100-1:500; Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
• Immunogen: Synthetic peptide corresponding to human UBA52 at C-terminal (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Prostate (formalin-fixed, paraffin-embedded) stained with UBA52 antibody followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Immunohistochemistry (IHC)

(Human Testis (formalin-fixed, paraffin-embedded) stained with UBA52 antibody followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-UBA52 antibody

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

Product Categories/Family for anti-UBA52 antibody

Antibodies; Abs to Ribosomal Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 77539055
• NCBI GeneID: 7311
• NCBI Accession #: NP_001029102.1
• NCBI GenBank Nucleotide #: NM_001033930.1
• UniProt Accession #: P62987; P02248; P02249; P02250; P14793; P62988; Q29120; Q6LBL4; Q6LDU5; Q8WYN8; Q91887; Q91888
• Molecular Weight: 14,728 Da
• NCBI Official Full Name: ubiquitin-60S ribosomal protein L40
• NCBI Official Synonym Full Names: ubiquitin A-52 residue ribosomal protein fusion product 1
• NCBI Official Symbol: UBA52
• NCBI Official Synonym Symbols: L40; CEP52; RPL40; HUBCEP52
• NCBI Protein Information: ubiquitin-60S ribosomal protein L40; ubiquitin-CEP52; ubiquitin-52 amino acid fusion protein; ubiquitin carboxyl extension protein 52
• UniProt Protein Name: Ubiquitin-60S ribosomal protein L40
• UniProt Gene Name: UBA52
• UniProt Synonym Gene Names: UBCEP2
• UniProt Entry Name: RL40_HUMAN

NCBI Description

Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Ubiquitin: exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Ref.8 Ref.12 Ref.1560S ribosomal protein L40: component of the 60S subunit of the ribosome. Ribosomal protein L40 is essential for translation of a subset of cellular transcripts, and especially for cap-dependent translation of vesicular stomatitis virus mRNAs. Ref.8 Ref.12 Ref.15

Subunit structure: Ribosomal protein L40 is part of the 60S ribosomal subunit

By similarity. Ref.15 Ref.17

Subcellular location: Ubiquitin: Cytoplasm

By similarity. Nucleus

By similarity. 60S ribosomal protein L40: Cytoplasm

By similarity.

Miscellaneous: Ubiquitin is encoded by 4 different genes. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains.For a better understanding, features related to ubiquitin are only indicated for the first chain.

Sequence similarities: In the N-terminal section; belongs to the ubiquitin family.In the C-terminal section; belongs to the ribosomal protein L40e family.Contains 1 ubiquitin-like domain.

Sequence caution: The sequence AAK31162.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.