Rabbit anti-Rat Tyrosine Hydroxylase Polyclonal Antibody | anti-TH antibody

Tyrosine Hydroxylase, phosphorylated (Ser40) (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase)

Cat. Num. AAA630964

• Gene Names: TH; TYH; DYT14; DYT5b
• Reactivity: Rat
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Tyrosine Hydroxylase; Polyclonal Antibody; phosphorylated (Ser40) (TH; TYH; Tyrosine 3 Hydroxylase; Tyrosine 3 Monooxygenase); Anti -Tyrosine Hydroxylase; anti-TH antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Tyrosine Hydroxylase, phosphoSer40. The antibody recognizes a protein of 60kD corresponding to TH phosphorylated at Ser40. Depending on brain region being studied, protein loads and detection methods used some higher molecular weight bands may be detected. Species Crossreactivity: Rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Dulbecco's phosphate-buffered saline (divalent-deficient) containing 10 mM HEPES (pH 7.2), 20 mM Tris-HCl (pH 7.6), 1 mg/mL BSA, 0.1% PVP-40, 0.05% Tween 20 and 0.1% sodium azide.

• Applicable Applications for anti-TH antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blotting, Immunoprecipitation.
• Immunogen: Synthetic peptide from the phosphoSer40 of rat Tyrosine Hydroxylase.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (30-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-TH antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 986909
• NCBI GeneID: 7054
• UniProt Accession #: O43816; P07101; P78428; Q0PWM2; Q0PWM3; Q15587; Q16846; Q2M3B4; Q6XS77; Q7KZ36
• Molecular Weight: 14,058 Da
• NCBI Official Full Name: tyrosine hydroxylase
• NCBI Official Synonym Full Names: tyrosine hydroxylase
• NCBI Official Symbol: TH
• NCBI Official Synonym Symbols: TYH; DYT14; DYT5b
• NCBI Protein Information: tyrosine 3-monooxygenase; dystonia 14; OTTHUMP00000011163; OTTHUMP00000011225; OTTHUMP00000011226; tyrosine 3-hydroxylase
• UniProt Protein Name: Tyrosine hydroxylase
• UniProt Entry Name: O43816_HUMAN

NCBI Description

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]

Uniprot Description

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.

Protein type: Endoplasmic reticulum; Vesicle; EC 1.14.16.2; Oxidoreductase; Mitochondrial; Amino Acid Metabolism - tyrosine

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: synaptic vesicle; neuron projection; internal side of plasma membrane; smooth endoplasmic reticulum; mitochondrion; melanosome membrane; cytoplasm; dendrite; terminal button; perikaryon; cytoplasmic vesicle; cytosol; nucleus

Molecular Function: amino acid binding; protein domain specific binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; tyrosine 3-monooxygenase activity

Biological Process: heart morphogenesis; heart development; response to lipopolysaccharide; dopamine biosynthetic process; phytoalexin metabolic process; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; catecholamine biosynthetic process; response to electrical stimulus; epinephrine biosynthetic process; neurotransmitter biosynthetic process; response to corticosterone stimulus; response to pyrethroid; response to light stimulus; anatomical structure morphogenesis; phthalate metabolic process; mating behavior; social behavior; organ morphogenesis; eye photoreceptor cell development; circadian sleep/wake cycle; response to ethanol; response to zinc ion; response to activity; cerebral cortex development; response to water deprivation; synaptic transmission, dopaminergic; response to peptide hormone stimulus; locomotory behavior; fatty acid metabolic process; regulation of heart contraction; response to salt stress; response to estradiol stimulus; sensory perception of sound; visual perception; glycoside metabolic process; response to nutrient levels; terpene metabolic process; sphingolipid metabolic process; eating behavior; response to amphetamine; multicellular organismal aging; isoquinoline alkaloid metabolic process; learning; response to herbicide; response to ether; synaptic vesicle amine transport; memory; pigmentation; response to hypoxia; embryonic camera-type eye morphogenesis

Disease: Segawa Syndrome, Autosomal Recessive