Rabbit anti-Human Tyk2 Polyclonal Antibody | anti-TYK2 antibody

Tyk2, phosphorylated (Tyr1054/1055) (Non-receptor Tyrosine Kinase)

Cat. Num. AAA610259

• Gene Names: TYK2; JTK1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Tyk2; Polyclonal Antibody; phosphorylated (Tyr1054/1055) (Non-receptor Tyrosine Kinase); Anti -Tyk2; anti-TYK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of Tyk2 only when activated by phosphorylation at tyrosine1054/1055. Does not detect other sites of tyrosine phosphorylation on Tyk2. Species Crossreactivity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Also available with BSA. See T9220-02A.

• Applicable Applications for anti-TYK2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding tyrosine 1054/1055 of human Tyk2. Molecular weight: 140kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of extracts from interferon-alpha treated HeLa cells, using MBS610259 (upper) or control Tyk2 antibody.)

Related Product Information for anti-TYK2 antibody

Tyk2, a member of the Jak family of protein tyrosine kinases, associates with and is activated by receptors for many cytokines including IL-13, the IL-6 family, IL-10, and interferon-alpha and beta (1-3). Following ligand binding, Tyk2 is activated by phosphorylation on Tyr1054 and/or Tyr1055 (4). Tyk2 is required for the tyrosine phosphorylation of Stat3 in the IFN-beta signaling cascade (5).

Product Categories/Family for anti-TYK2 antibody

Antibodies; Abs to Tyrosine Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 42716354
• NCBI GeneID: 7297
• UniProt Accession #: P29597; Q6QB10; Q96CH0; Q53HA9
• Molecular Weight: 133,650 Da
• NCBI Official Full Name: tyrosine kinase 2
• NCBI Official Synonym Full Names: tyrosine kinase 2
• NCBI Official Symbol: TYK2
• NCBI Official Synonym Symbols: JTK1
• NCBI Protein Information: non-receptor tyrosine-protein kinase TYK2; OTTHUMP00000232745; OTTHUMP00000232746; OTTHUMP00000232748
• UniProt Protein Name: Non-receptor tyrosine-protein kinase TYK2
• Protein Family: Non-receptor tyrosine-protein kinase
• UniProt Gene Name: TYK2
• UniProt Entry Name: TYK2_HUMAN

NCBI Description

This gene encodes a member of the tyrosine kinase and, more specifically, the Janus kinases (JAKs) protein families. This protein associates with the cytoplasmic domain of type I and type II cytokine receptors and promulgate cytokine signals by phosphorylating receptor subunits. It is also component of both the type I and type III interferon signaling pathways. As such, it may play a role in anti-viral immunity. A mutation in this gene has been associated with hyperimmunoglobulin E syndrome (HIES) - a primary immunodeficiency characterized by elevated serum immunoglobulin E. [provided by RefSeq]

Uniprot Description

Tyk2: a non-receptor tyrosine-protein kinase of the JakA family. Activated in signaling from the IL-2, gp130 and IFN families of receptors. Phosphorylates the interferon-alpha/beta receptor alpha chain.

Protein type: Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; TK group; JakA family

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; growth hormone receptor binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; type 1 angiotensin receptor binding; ATP binding

Biological Process: cell migration; cytokine and chemokine mediated signaling pathway; innate immune response; cell differentiation; protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation

Disease: Immunodeficiency 35