Rabbit anti-Human PODXL2 Polyclonal Antibody | anti-PODXL2 antibody

PODXL2 Antibody

Cat. Num. AAA9603029

• Gene Names: PODXL2; EG; PODLX2
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: Peptide affinity purification
• Synonyms: PODXL2; Polyclonal Antibody; PODXL2 Antibody; EG; OTTHUMP00000216064; PODLX2; podocalyxin like 2; Podocalyxin like protein 2; anti-PODXL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PODXL2 antibody detects endogenous levels of total PODXL2
• Purity/Purification: Peptide affinity purification
• Form/Format: IgG phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 605

• Applicable Applications for anti-PODXL2 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:1000-3000; IF/ICC: 1:100-1:500; ELISA: 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human PODXL2, corresponding to a region within C-Terminal amino acids.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of PODXL2 using COLO205 whole cell lysates)

Immunofluorescene (IF)

(MBS9603029 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Related Product Information for anti-PODXL2 antibody

Function: Acts as a ligand for vascular selectins. Mediates rapid rolling of leukocytes over vascular surfaces through high affinity divalent cation-dependent interactions with E-, P-and L-selectins.
Subunit Structure: Homodimer; disulfide-linked. Interacts with SELL, SELE and SELP.
Post-translational Modifications: Glycosylated; contains chondroitin sulfate. Displays sialylated O-linked oligosaccharides. Sulfation is necessary for interaction with SELL. Sialylated O-linked oligosaccharides are necessary for interaction with SELL, SELE and SELP.
Similarity: Belongs to the podocalyxin family.

NCBI and Uniprot Product Information

• NCBI GI #: 7657465
• NCBI GeneID: 50512
• NCBI Accession #: NP_056535.1
• NCBI GenBank Nucleotide #: NM_015720.3
• UniProt Accession #: Q9NZ53; Q6UVY4; Q8WUV6
• Molecular Weight: 65kDa
• NCBI Official Full Name: podocalyxin-like protein 2
• NCBI Official Synonym Full Names: podocalyxin like 2
• NCBI Official Symbol: PODXL2
• NCBI Official Synonym Symbols: EG; PODLX2
• NCBI Protein Information: podocalyxin-like protein 2
• UniProt Protein Name: Podocalyxin-like protein 2
• Protein Family: Podocalyxin-like protein
• UniProt Gene Name: PODXL2

NCBI Description

This gene is a member of the CD34 family of cell surface transmembrane proteins, which are characterized by an N-terminal extracellular mucin domain, globular and stalk domains, a single pass transmembrane region, and a charged cytoplasmic tail. The encoded protein is a ligand for vascular selectins. [provided by RefSeq, Oct 2012]

Uniprot Description

Acts as a ligand for vascular selectins. Mediates rapid rolling of leukocytes over vascular surfaces through high affinity divalent cation-dependent interactions with E-, P- and L-selectins.