Goat anti-Human TXNRD1 Polyclonal Antibody | anti-TXNRD1 antibody

TXNRD1, CT (Thioredoxin Reductase 1, Cytoplasmic, TR, Gene Associated with Retinoic and Interferon-induced Mortality 12 Protein, GRIM-12, Gene Associated with Retinoic and IFN-induced Mortality 12 Protein, KM-102-derived Reductase-like Factor, Thioredoxin

Cat. Num. AAA6003328

• Gene Names: TXNRD1; TR; TR1; TXNR; TRXR1; GRIM-12
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: TXNRD1; Polyclonal Antibody; CT (Thioredoxin Reductase 1; Cytoplasmic; TR; Gene Associated with Retinoic and Interferon-induced Mortality 12 Protein; GRIM-12; Gene Associated with Retinoic and IFN-induced Mortality 12 Protein; KM-102-derived Reductase-like Factor; Thioredoxin; Anti -TXNRD1; anti-TXNRD1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human TXNRD1. Species sequence homology: Porcine.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-TXNRD1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 32,000; Western Blot: 1:32,000 & 0.1-1ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml
• Immunogen: Synthetic peptide corresponding to TKRSGASILQAGC from C-terminus of human TXNRD1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC)

(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Antibody staining (0.1ug/ml) of Human Placenta lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

Related Product Information for anti-TXNRD1 antibody

The mammalian thioredoxin reductases (TrxRs) are a family of selenocysteine-containing pyridine nucleotide-disulfide oxido-reductases. All the mammalian TrxRs are homologous to glutathione reductase with respect to primary structure including the conserved redox catalytic site (-Cys-Val-Asn-Val-Gly-Cys-) but distinctively with a C-terminal extension containing a catalytically active penultimate selenocysteine (SeCys) residue in the conserved sequence(-Gly-Cys-SeCys-Gly). TrxR is homodimeric protein in which each monomer includes an FAD prosthetic group, a NADPH binding site and a redox catalytic site. Electrons are transferred from NADPH via FAD and the active-site disulfide to C-terminal SeCys-containing redox center, which then reduces the substrate like thioredoxin. The members of TrxR family are 55-58 kilodalton in molecular size and composed of three isoforms including cytosolic TrxR1,mitochondrial TrxR2, and TrxR3, known as Trx and GSSG reductase (TGR). TrxR plays a key role in protection of cells against oxidative stress and redox-regulatory mechanism of transcription factors and varioMyBioSource phenomena.

Product Categories/Family for anti-TXNRD1 antibody

Antibodies; Abs to Thioredoxin

NCBI and Uniprot Product Information

• NCBI GI #: 387598071
• NCBI GeneID: 7296
• NCBI Accession #: NP_001248375.1
• NCBI GenBank Nucleotide #: NM_001261446.1
• UniProt Accession #: Q16881; Q6FI31; Q6VB40; Q6VB41; Q6VB42; Q6VBP2; Q6VBP3; Q6VBP4; Q6VBP5; Q6VBP9; Q6VBQ0; Q6YNQ1
• Molecular Weight: 70,906 Da
• NCBI Official Full Name: thioredoxin reductase 1, cytoplasmic isoform 5
• NCBI Official Synonym Full Names: thioredoxin reductase 1
• NCBI Official Symbol: TXNRD1
• NCBI Official Synonym Symbols: TR; TR1; TXNR; TRXR1; GRIM-12
• NCBI Protein Information: thioredoxin reductase 1, cytoplasmic; oxidoreductase; thioredoxin reductase TR1; thioredoxin reductase GRIM-12; KM-102-derived reductase-like factor; gene associated with retinoic and IFN-induced mortality 12 protein; gene associated with retinoic and interferon-induced mortality 12 protein
• UniProt Protein Name: Thioredoxin reductase 1, cytoplasmic
• Protein Family: Thioredoxin reductase
• UniProt Gene Name: TXNRD1
• UniProt Synonym Gene Names: GRIM12; KDRF; TR; GRIM-12
• UniProt Entry Name: TRXR1_HUMAN

NCBI Description

This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

TRXR1: a cytoplasmic pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine residue which is required for the catalytic activity.

Protein type: Oxidoreductase; EC 1.8.1.9; Nucleotide Metabolism - pyrimidine; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 12q23-q24.1

Cellular Component: nucleoplasm; nucleolus; cytosol

Molecular Function: protein binding; thioredoxin-disulfide reductase activity; FAD binding; electron carrier activity; protein disulfide oxidoreductase activity

Biological Process: response to reactive oxygen species; nucleobase, nucleoside and nucleotide metabolic process; cell redox homeostasis; nucleobase, nucleoside and nucleotide interconversion; cellular lipid metabolic process; signal transduction