Western Blot (WB)
(The anti-TrxL Pab is used in Western blot to detect TrxL in HL-60 cell lysate.)
Rabbit TXNL1 (TRP32/TrxL) Polyclonal Antibody | anti-TXNL1 antibody
TXNL1 (TRP32/TrxL) Antibody (C-term)
Gene Names
TXNL1; Txl; TXNL; TRP32; TXL-1; HEL-S-114
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TXNL1 (TRP32/TrxL); Polyclonal Antibody; TXNL1 (TRP32/TrxL) Antibody (C-term); Thioredoxin-like protein 1; 32 kDa thioredoxin-related protein; TXNL1; TRP32; TXL; TXNL; anti-TXNL1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TXNL1 (TRP32/TrxL) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-289 amino acids from the C-terminal region of human TXNL1 (TRP32/TrxL).
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
256-289
Sequence Length
289
Applicable Applications for anti-TXNL1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(The anti-TrxL Pab is used in Western blot to detect TrxL in HL-60 cell lysate.)
Western Blot (WB)
(The anti-TrxL Pab is used in Western blot to detect TrxL in HL-60 cell lysate.)
Immunohistochemistry (IHC)
(Mouse cerebellar cortex showing molecular cell layer (mc), Purkinje cells (Pc) and granular cell layer. Trxl-1 antibody. The results correspond precisely to TrxL-1 mRNA expression shown with in situ hybridization (Jim nez A et al. FEBS Lett. 2006, 580(3):960-7) and could be abolished with peptide used for immunization (Blocking peptide). The fixation was 4% paraformaldehyde perfusion (4mins) and 60 mins immersion. Staining was done in frozen sections with Vector Elite kit and nickel intensified DAB as a chromogen. Data courtesy of Prof. Markku Pelto-Huikko,Department of Developmental Biology, Medical School,Tampere University, Finland.)
Immunohistochemistry (IHC)
(Mouse cerebellar cortex showing molecular cell layer (mc), Purkinje cells (Pc) and granular cell layer. Trxl-1 antibody. The results correspond precisely to TrxL-1 mRNA expression shown with in situ hybridization (Jim nez A et al. FEBS Lett. 2006, 580(3):960-7) and could be abolished with peptide used for immunization (Blocking peptide). The fixation was 4% paraformaldehyde perfusion (4mins) and 60 mins immersion. Staining was done in frozen sections with Vector Elite kit and nickel intensified DAB as a chromogen. Data courtesy of Prof. Markku Pelto-Huikko,Department of Developmental Biology, Medical School,Tampere University, Finland.)
Related Product Information for anti-TXNL1 antibody
Thioredoxins are a group of small redox active proteins possessing a conserved active site sequence. TrxL (Thioredoxin like protein 1) has 2 distinct domains: an N terminal domain, which is 43% identical to human TXN, and a C terminal domain, which shows no homology to other proteins in the sequence databases. The active site sequence, located within the N terminal domain, is that of a thioredoxin like protein; compared to the active site sequence of TXN, it has a single amino acid substitution. Unlike other thioredoxin like proteins, TrxL does not serve as a substrate for thioredoxin reductase in an insulin assay.
Product Categories/Family for anti-TXNL1 antibody
References
Miranda-Vizuete, A., et al., Biochem. Biophys. Res. Commun. 243: 284-288 (1998).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32251
NCBI Official Full Name
thioredoxin-like protein 1
NCBI Official Synonym Full Names
thioredoxin-like 1
NCBI Official Symbol
TXNL1
NCBI Official Synonym Symbols
Txl; TXNL; TRP32; TXL-1; HEL-S-114
NCBI Protein Information
thioredoxin-like protein 1
UniProt Protein Name
Thioredoxin-like protein 1
Protein Family
UniProt Gene Name
TXNL1
UniProt Synonym Gene Names
TRP32; TXL; TXNL
UniProt Entry Name
TXNL1_HUMAN
Uniprot Description
TXNL1: a thiol reductase and novel subunit of the proteasome 19S particle. Interconverts reduced sulfide groups and oxidized disulfide bonds in a substrate.
Protein type: Oxidoreductase
Chromosomal Location of Human Ortholog: 18q21.31
Cellular Component: proteasome complex; cytoplasm; nucleus
Molecular Function: disulfide oxidoreductase activity; protein disulfide oxidoreductase activity
Biological Process: cell redox homeostasis; glycerol ether metabolic process
Research Articles on TXNL1
Similar Products
Product Notes
The TXNL1 txnl1 (Catalog #AAA9201064) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 256-289. The TXNL1 (TRP32/TrxL) Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's TXNL1 (TRP32/TrxL) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the TXNL1 txnl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TXNL1 (TRP32/TrxL), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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