Western Blot (WB)
(Western Blot analysis of TXNIP on different lysates using anti-TXNIP antibody at 1/1,000 dilution. Positive control: Lane 1: PC-12 Lane 2: Human skeletal muscle Lane 3: NIH-3T3 Lane 4: Hela)
Rabbit TXNIP Polyclonal Antibody | anti-TXNIP antibody
TXNIP Polyclonal Antibody
ICC: 1:50-1:200
IF: 1:50-1:200
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of TXNIP on different lysates using anti-TXNIP antibody at 1/1,000 dilution. Positive control: Lane 1: PC-12 Lane 2: Human skeletal muscle Lane 3: NIH-3T3 Lane 4: Hela)
Western Blot (WB)
(Western Blot analysis of TXNIP on different lysates using anti-TXNIP antibody at 1/1,000 dilution. Positive control: Lane 1: PC-12 Lane 2: Human skeletal muscle Lane 3: NIH-3T3 Lane 4: Hela)
Immunocytochemistry (ICC)
(ICC staining TXNIP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining TXNIP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a thioredoxin-binding protein that is a member of the alpha arrestin protein family. Thioredoxin is a thiol-oxidoreductase that is a major regulator of cellular redox signaling which protects cells from oxidative stress. This protein inhibits the antioxidative function of thioredoxin resulting in the accumulation of reactive oxygen species and cellular stress. This protein also functions as a regulator of cellular metabolism and of endoplasmic reticulum (ER) stress. This protein may also function as a tumor suppressor. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Uniprot Description
TXNIP: May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over- expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth. Belongs to the arrestin family.
Protein type: Tumor suppressor; Motility/polarity/chemotaxis; Mitochondrial; Apoptosis
Chromosomal Location of Human Ortholog: 1q21.1
Cellular Component: cytoplasm; mitochondrial intermembrane space; cytosol; nucleus
Molecular Function: enzyme inhibitor activity; protein binding; ubiquitin protein ligase binding
Biological Process: response to drug; transcription, DNA-dependent; positive regulation of apoptosis; platelet-derived growth factor receptor signaling pathway; negative regulation of cell division; negative regulation of transcription from RNA polymerase II promoter; cell cycle; response to estradiol stimulus; regulation of cell proliferation; keratinocyte differentiation; response to hydrogen peroxide; response to mechanical stimulus; protein import into nucleus; negative regulation of catalytic activity; response to glucose stimulus; innate immune response; response to calcium ion; response to progesterone stimulus
Research Articles on TXNIP
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Product Notes
The TXNIP txnip (Catalog #AAA3013620) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TXNIP Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TXNIP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1000-1:5,000 ICC: 1:50-1:200 IF: 1:50-1:200 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the TXNIP txnip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TXNIP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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