Rabbit anti-Human TXNIP Polyclonal Antibody | anti-TXNIP antibody

TXNIP, NT (TXNIP, VDUP1, Thioredoxin-interacting protein, Thioredoxin-binding protein 2, Vitamin D3 up-regulated protein 1) (APC)

Cat. Num. AAA6360388

• Gene Names: TXNIP; THIF; VDUP1; HHCPA78; EST01027
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TXNIP; Polyclonal Antibody; NT (TXNIP; VDUP1; Thioredoxin-interacting protein; Thioredoxin-binding protein 2; Vitamin D3 up-regulated protein 1) (APC); Vitamin D3 up-regulated protein 1; anti-TXNIP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-TXNIP antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TXNIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TXNIP.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TXNIP Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the TXNIP antibody detected the TXNIP protein (arrow).)

Related Product Information for anti-TXNIP antibody

TXNIP may act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells.

Product Categories/Family for anti-TXNIP antibody

Antibodies; Binding Proteins

References

Zhuo de, X., et al. J. Biol. Chem. 285(41):31491-31501(2010). Kwon, H.J., et al. J. Immunol. 185(7):3980-3989(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Yu, F.X., et al. J. Biol. Chem. 285(33):25822-25830(2010). Cadenas, C., et al. Breast Cancer Res. 12 (3), R44 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 171184421
• NCBI GeneID: 10628
• NCBI Accession #: NP_006463.3
• NCBI GenBank Nucleotide #: NM_006472.4
• UniProt Accession #: Q9H3M7; Q16226; Q6PML0; Q9BXG9; B4E3D3
• Molecular Weight: 43,661 Da
• NCBI Official Full Name: thioredoxin-interacting protein
• NCBI Official Synonym Full Names: thioredoxin interacting protein
• NCBI Official Symbol: TXNIP
• NCBI Official Synonym Symbols: THIF; VDUP1; HHCPA78; EST01027
• NCBI Protein Information: thioredoxin-interacting protein; thioredoxin binding protein 2; thioredoxin-binding protein 2; vitamin D3 up-regulated protein 1; upregulated by 1,25-dihydroxyvitamin D-3
• UniProt Protein Name: Thioredoxin-interacting protein
• Protein Family: Thioredoxin-interacting protein
• UniProt Gene Name: TXNIP
• UniProt Synonym Gene Names: VDUP1
• UniProt Entry Name: TXNIP_HUMAN

Uniprot Description

TXNIP: May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over- expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth. Belongs to the arrestin family.

Protein type: Tumor suppressor; Motility/polarity/chemotaxis; Mitochondrial; Apoptosis

Chromosomal Location of Human Ortholog: 1q21.1

Cellular Component: cytoplasm; mitochondrial intermembrane space; cytosol; nucleus

Molecular Function: enzyme inhibitor activity; protein binding; ubiquitin protein ligase binding

Biological Process: response to drug; transcription, DNA-dependent; positive regulation of apoptosis; platelet-derived growth factor receptor signaling pathway; negative regulation of cell division; negative regulation of transcription from RNA polymerase II promoter; cell cycle; response to estradiol stimulus; regulation of cell proliferation; keratinocyte differentiation; response to hydrogen peroxide; response to mechanical stimulus; protein import into nucleus; negative regulation of catalytic activity; response to glucose stimulus; innate immune response; response to calcium ion; response to progesterone stimulus