Mouse anti-Human TXNDC3 Polyclonal Antibody | anti-NME8 antibody

TXNDC3 (Thioredoxin Domain-containing Protein 3, CILD6, NM23-H8, NME/NM23 Family Member 8, NME8, Spermatid-specific Thioredoxin-2, SPTRX2, Sptrx-2)

Cat. Num. AAA642778

• Gene Names: NME8; CILD6; SPTRX2; TXNDC3; NM23-H8; sptrx-2
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: TXNDC3; Polyclonal Antibody; TXNDC3 (Thioredoxin Domain-containing Protein 3; CILD6; NM23-H8; NME/NM23 Family Member 8; NME8; Spermatid-specific Thioredoxin-2; SPTRX2; Sptrx-2); Anti -TXNDC3 (Thioredoxin Domain-containing Protein 3; anti-NME8 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TXNDC3.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MASKKREVQLQTVINNQSLWDEMLQNKGLTVIDVYQAWCGPCRAMQPLFRKLKNELNEDEILHFAVAEADNIVTLQPFRDKCEPVFLFSVNGKIIEKIQGANAPLVNKKVINLIDEERKIAAGEMARPQYPEIPLVDSDSEVSEESPCESVQELYSIAIIKPDAVISKKVLEIKRKITKAGFIIEAEHKTVLTEEQVVNFYSRIADQRDFEEFVSFMTSGLSYILVVSQGSKHNPPSEETEPQTDTEPNERSEDQPEVEAQVTPGMMKNKQDSLQEYLERQHLAQLCDIEEDAANVAKFMDAFFPDFKKMKSMKLEKTLALLRPNLFHERKDDVLRIIKDEDFKILEQRQVVLSEKEAQALCKEYENEDYFNKLIENMTSGPSLALVLLRDNGLQYWKQLLGPRTVEEAIEYFPESLCAQFAMDSLPVNQLYGSDSLETAEREIQHFFPLQSTLGLIKPHATSEQREQILKIVKEAGFDLTQVKKMFLTPEQTEKIYPKVTGKDFYKDLLEMLSVGPSMVMILTKWNAVAEWRRLMGPTDPEEAKLLSPDSIRAQFGISKLKNIVHGASNAYEAKEVVNRLFEDPEEN

• Applicable Applications for anti-NME8 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Western Blot.; Dilution: Immunofluorescence: 10ug/ml
• Immunogen: Full length human TXNDC3, aa1-588 (NP_057700.2).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of TXNDC3 expression in transfected 293T cell line by TXNDC3 polyclonal antibody. Lane 1: TXNDC3 transfected lysate (64.68kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of purified antibody to TXNDC3 on HeLa cell. [antibody concentration 10ug/ml])

Product Categories/Family for anti-NME8 antibody

Antibodies; Abs to Thioredoxin

NCBI and Uniprot Product Information

• NCBI GI #: 148839372
• NCBI GeneID: 51314
• NCBI Accession #: NP_057700.3
• NCBI GenBank Nucleotide #: NM_016616.4
• UniProt Accession #: Q8N427; Q9NZH1
• Molecular Weight: 67,270 Da
• NCBI Official Full Name: thioredoxin domain-containing protein 3
• NCBI Official Synonym Full Names: NME/NM23 family member 8
• NCBI Official Symbol: NME8
• NCBI Official Synonym Symbols: CILD6; SPTRX2; TXNDC3; NM23-H8; sptrx-2
• NCBI Protein Information: thioredoxin domain-containing protein 3; sperm-specific thioredoxin 2; spermatid-specific thioredoxin-2; thioredoxin domain containing 3 (spermatozoa)
• UniProt Protein Name: Thioredoxin domain-containing protein 3
• Protein Family: Thioredoxin domain-containing protein
• UniProt Gene Name: NME8
• UniProt Synonym Gene Names: SPTRX2; TXNDC3; Sptrx-2
• UniProt Entry Name: TXND3_HUMAN

NCBI Description

This gene encodes a protein with an N-terminal thioredoxin domain and three C-terminal nucleoside diphosphate kinase (NDK) domains, but the NDK domains are thought to be catalytically inactive. The sea urchin ortholog of this gene encodes a component of sperm outer dynein arms, and the protein is implicated in ciliary function. Mutations in this gene are implicated in primary ciliary dyskinesia type 6.[provided by RefSeq, Nov 2009]

Uniprot Description

TXNDC3: Probably required during the final stages of sperm tail maturation in the testis and/or epididymis, where extensive disulfide bonding of fibrous sheath (FS) proteins occurs. May be involved in the reduction of disulfide bonds within the sperm FS components. In vitro, it has neither NDP kinase nor reducing activity on disulfide bonds. Defects in NME8 are the cause of primary ciliary dyskinesia type 6 (CILD6). CILD is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome.

Protein type: Kinase, nucleoside diphosphate; Oxidoreductase; Other group; NDK family

Chromosomal Location of Human Ortholog: 7p14.1

Cellular Component: cytoplasm; intracellular

Molecular Function: nucleoside diphosphate kinase activity; ATP binding

Biological Process: GTP biosynthetic process; CTP biosynthetic process; cell redox homeostasis; multicellular organismal development; UTP biosynthetic process; nucleoside diphosphate phosphorylation; spermatogenesis; cell differentiation

Disease: Ciliary Dyskinesia, Primary, 6