VCAM1 recombinant protein

VCAM1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8118835

• Gene Names: Vcam1; CD106; Vcam-1
• Purity: >97 % as determined by SDS-PAGE
• Synonyms: VCAM1; VCAM1 Protein; Mouse; Recombinant (His Tag); Mouse VCAM1/L1CAM/CD106 Protein (His Tag); vascular cell adhesion molecule 1; CD106 Protein; Vcam-1 Protein; VCAM1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4 Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 685aa
• Sequence: Met1-Glu698

• Species: Mouse
• Tag: C-His
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Phe25
• Protein Construction: |A DNA sequence encoding the extracellular domain (Met 1-Glu 698) of mouse VCAM1 (NP_035823.3) was fused with the a polyhistidine tag at the C-terminus
• Activity: Measured by the ability of the immobilized protein to support adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to mouse VCAM1 coated plates (10ug/ml with 100ul/well), approximately 70%-80% cells will adhere after 1 hour at RT.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Related Product Information for VCAM1 recombinant protein

Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing, with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and alpha4beta7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.

References

Cook-Mills JM. (2002) VCAM-1 signals during lymphocyte migration: role of reactive oxygen species. Mol Immunol. 39(9): 499-508.
Preiss DJ, et al. (2007) Vascular cell adhesion molecule-1: a viable therapeutic target for atherosclerosis? Int J Clin Pract. 61(4): 697-701.

NCBI and Uniprot Product Information

• NCBI GI #: 170295823
• NCBI GeneID: 22329
• NCBI Accession #: NP_035823.3
• NCBI GenBank Nucleotide #: NM_011693.3
• UniProt Accession #: P29533
• Molecular Weight: 38,372 Da
• NCBI Official Full Name: vascular cell adhesion protein 1
• NCBI Official Synonym Full Names: vascular cell adhesion molecule 1
• NCBI Official Symbol: Vcam1
• NCBI Official Synonym Symbols: CD106; Vcam-1
• NCBI Protein Information: vascular cell adhesion protein 1
• UniProt Protein Name: Vascular cell adhesion protein 1
• Protein Family: Vascular cell adhesion protein
• UniProt Gene Name: Vcam1
• UniProt Synonym Gene Names: Vcam-1; V-CAM 1; VCAM-1
• UniProt Entry Name: VCAM1_MOUSE

Uniprot Description

VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Cellular Component: apical part of cell; cell surface; early endosome; endoplasmic reticulum; external side of plasma membrane; extracellular space; filopodium; Golgi apparatus; microvillus; podosome; sarcolemma

Molecular Function: amine oxidase activity; cell adhesion molecule binding; integrin binding

Biological Process: amine metabolic process; cell adhesion; cell-matrix adhesion; heart development; heterophilic cell adhesion; leukocyte adhesion; leukocyte tethering or rolling; positive regulation of T cell proliferation