Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Thymosin beta-10 (TMSB10) Standard Curve.)
Human Thymosin beta-10 (TMSB10) ELISA Kit | TMSB10 elisa kit
Human Thymosin beta-10 (TMSB10) ELISA Kit
Gene Names
TMSB10; TB10; MIG12
Reactivity
Human
Synonyms
Thymosin beta-10 (TMSB10); Human Thymosin beta-10 (TMSB10) ELISA Kit; TMSB10; MIG12; TB10; migration-inducing gene 12; migration-inducing protein 12; thymosin; beta 10; TMSB10 elisa kit
Reactivity
Human
Specificity
Human Thymosin beta-10 (TMSB10) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMSB10. No significant cross-reactivity or interference between Human TMSB10 and analogues was observed.
Sequence Length
44
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Thymosin beta-10 (TMSB10) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Thymosin beta-10 (TMSB10) Standard Curve.)
Related Product Information for TMSB10 elisa kit
Description: This Human Thymosin beta-10 (TMSB10) ELISA Kit employs a two-site sandwich ELISA to quantitate TMSB10 in samples. An antibody specific for TMSB10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMSB10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMSB10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMSB10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Thymosin-beta-10 is closely related in sequence to TMSB4 and is also an actin-sequestering protein. In most tissues of the rat, the same molecular weight species of TMSB10 mRNA is found; This finding, together with primer extension experiments, suggested that the 2 mRNAs are transcribed from the same gene through a combination of differential promoter utilization and alternative splicing. Both mRNAs were present in pachytene spermatocytes; only the testis-specific mRNA was detected in postmeiotic haploid spermatids. Immunohistochemical analysis showed that the protein was present in differentiating spermatids, suggesting that the testis-specific TMSB10 mRNA is translated in haploid male germ cells. thymosin-beta-10 protein synthesized in adult testis was identical in size to that synthesized in brain.
Background: Thymosin-beta-10 is closely related in sequence to TMSB4 and is also an actin-sequestering protein. In most tissues of the rat, the same molecular weight species of TMSB10 mRNA is found; This finding, together with primer extension experiments, suggested that the 2 mRNAs are transcribed from the same gene through a combination of differential promoter utilization and alternative splicing. Both mRNAs were present in pachytene spermatocytes; only the testis-specific mRNA was detected in postmeiotic haploid spermatids. Immunohistochemical analysis showed that the protein was present in differentiating spermatids, suggesting that the testis-specific TMSB10 mRNA is translated in haploid male germ cells. thymosin-beta-10 protein synthesized in adult testis was identical in size to that synthesized in brain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
thymosin beta-10
NCBI Official Synonym Full Names
thymosin beta 10
NCBI Official Symbol
TMSB10
NCBI Official Synonym Symbols
TB10; MIG12
NCBI Protein Information
thymosin beta-10
UniProt Protein Name
Thymosin beta-10
Protein Family
UniProt Gene Name
TMSB10
UniProt Synonym Gene Names
PTMB10; THYB10
UniProt Entry Name
TYB10_HUMAN
Uniprot Description
TMSB10: Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Belongs to the thymosin beta family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 2p11.2
Cellular Component: cytoskeleton; cytoplasm
Molecular Function: actin monomer binding
Biological Process: sequestering of actin monomers; actin filament organization
Research Articles on TMSB10
Similar Products
Product Notes
The Human TMSB10 tmsb10 (Catalog #AAA9713919) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713919 ELISA Kit recognizes Human TMSB10. It is sometimes possible for the material contained within the vial of "Thymosin beta-10 (TMSB10), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
