Goat anti-Human TXK / RLK Polyclonal Antibody | anti-TXK antibody

Goat Polyclonal to Human TXK / RLK

Cat. Num. AAA248727

• Gene Names: TXK; RLK; TKL; BTKL; PTK4; PSCTK5
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: TXK / RLK; Polyclonal Antibody; Goat Polyclonal to Human TXK / RLK; Anti-TXK / RLK Antibody (C-Terminus) IHC-plus; TXK; PSCTK5; PTK4 protein tyrosine kinase 4; Resting lymphocyte kinase; RLK; TKL; Tyrosine kinase; Tyrosine-protein kinase TXK; Protein-tyrosine kinase 4; BTKL; PTK4; TXK tyrosine kinase; Human TXK; anti-TXK antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human TXK.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Sequence Length: 527

• Applicable Applications for anti-TXK antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:4000), IHC-P (5 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Preliminary experiments gave an approx. 75kDa band in lysates of cell lines Jurkat and MOLT4 after 1 ug/ml antibody staining. Please note that currently we cannot find an explanat...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-ELLRAVTEIAETW from the C-terminus of human TXK (NP_003319.2). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Gibbon (92%), Monkey (85%).
• Immunogen Type: Synthetic peptide
• Immunogen: TXK / RLK antibody was raised against synthetic peptide C-ELLRAVTEIAETW from the C-terminus of human TXK (NP_003319.2). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Gibbon (92%), Monkey (85%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-TXK / RLK antibody IHC staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-TXK / RLK antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Related Product Information for anti-TXK antibody

TXK is a member of the BTK/TEC subfamily of non-receptor protein-tyrosine kinases. Unlike the other BTK/TEC family members, TXK has a unique proline-rich N-terminus that does not contain a pleckstrin homology domain. Txk lacks a PH domain and instead possesses a palmitoylated cysteine-string motif. Txk is phosphorylated in response to T-cell receptor stimulation and can be activated by phosphorylation by Src family kinases.

Product Categories/Family for anti-TXK antibody

Family: Protein Kinase; Subfamily: BTK/TEC

NCBI and Uniprot Product Information

• NCBI GI #: 148596974
• NCBI GeneID: 7294
• NCBI Accession #: NP_003319.2
• NCBI GenBank Nucleotide #: NM_003328.2
• UniProt Accession #: P42681; Q14220
• Molecular Weight: 61,258 Da
• NCBI Official Full Name: tyrosine-protein kinase TXK
• NCBI Official Synonym Full Names: TXK tyrosine kinase
• NCBI Official Symbol: TXK
• NCBI Official Synonym Symbols: RLK; TKL; BTKL; PTK4; PSCTK5
• NCBI Protein Information: tyrosine-protein kinase TXK; tyrosine kinase; protein-tyrosine kinase 4; resting lymphocyte kinase; PTK4 protein tyrosine kinase 4
• UniProt Protein Name: Tyrosine-protein kinase TXK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: TXK
• UniProt Synonym Gene Names: PTK4; RLK
• UniProt Entry Name: TXK_HUMAN

Uniprot Description

TXK: Non-receptor tyrosine kinase that plays a redundant role with ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T- cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of TXK to the cell membrane, where it is phosphorylated at Tyr-420. Phosphorylation leads to TXK full activation. Contributes also to signaling from many receptors and participates in multiple downstream pathways, including regulation of the actin cytoskekleton. Like ITK, can phosphorylate PLCG1, leading to its localization in lipid rafts and activation, followed by subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. With PARP1 and EEF1A1, TXK forms a complex that acts as a T-helper 1 (Th1) cell- specific transcription factor and binds the promoter of IFNG to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Phosphorylates both PARP1 and EEF1A1. Phosphorylates also key sites in LCP2 leading to the up-regulation of Th1 preferred cytokine IL-2. Phosphorylates 'Tyr- 201' of CTLA4 which leads to the association of PI-3 kinase with the CTLA4 receptor. Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.

Protein type: Protein kinase, TK; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Kinase, protein; TK group; Tec family

Chromosomal Location of Human Ortholog: 4p12

Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm; nucleus

Molecular Function: protein binding; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: adaptive immune response; transcription, DNA-dependent; protein amino acid autophosphorylation; cytokine production; T cell receptor signaling pathway; protein amino acid phosphorylation; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; interleukin-4 production; B cell receptor signaling pathway; positive regulation of interferon-gamma production; phospholipase C activation; interferon-gamma production; positive regulation of transcription from RNA polymerase II promoter; NK T cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; T cell differentiation