Rabbit TWEAK Polyclonal Antibody | anti-TWEAK antibody

TWEAK Antibody

Cat. Num. AAA9401902

• Gene Names: TNFSF12; APO3L; DR3LG; TWEAK
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified IgG
• Synonyms: TWEAK; Polyclonal Antibody; TWEAK Antibody; TNF-related weak inducer of apoptosis; TNF ligand superfamily member 12; anti-TWEAK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Purified IgG
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 249

• Applicable Applications for anti-TWEAK antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Recombinant protein
• Immunogen Description: Raised against recombinant human TWEAK.
• Target Name: TWEAK
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of (A) 5 ng, (B) 25 ng and (C) 50 ng of recombinant TWEAK with TWEAK antibody at 1 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of TWEAK in human brain tissue with TWEAK antibody at 10 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of TWEAK in mouse brain tissue with TWEAK antibody at 5 ug/mL.)

Related Product Information for anti-TWEAK antibody

TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane. TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, in addition to stimulation of apoptosis. The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains.

Product Categories/Family for anti-TWEAK antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4507597
• NCBI GeneID: 8742
• NCBI Accession #: NP_003800
• NCBI GenBank Nucleotide #: NM_003809.2
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 12 proprotein
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 12
• NCBI Official Symbol: TNFSF12
• NCBI Official Synonym Symbols: APO3L; DR3LG; TWEAK
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 12
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 12
• UniProt Gene Name: TNFSF12
• UniProt Synonym Gene Names: APO3L; DR3LG; TWEAK
• UniProt Entry Name: TNF12_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine, which exists in both membrane-bound and secreted forms, can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. Alternative splicing results in multiple transcript variants. Some transcripts skip the last exon of this gene and continue into the second exon of the neighboring TNFSF13 gene; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010]

Uniprot Description

TNFSF12: Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Homotrimer (Potential). Interacts with the angiogenic factor AGGF1/VG5Q. Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain. Belongs to the tumor necrosis factor family.

Protein type: Cytokine; Motility/polarity/chemotaxis; Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: extracellular space; perinuclear region of cytoplasm; integral to plasma membrane

Molecular Function: protein binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: positive regulation of angiogenesis; apoptosis; positive regulation of protein catabolic process; endothelial cell migration; immune response; positive regulation of endothelial cell proliferation; angiogenesis; cell differentiation; signal transduction