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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Human Tumor Necrosis Factor Ligand Superfamily, Member 7 (TNFSF7) Polyclonal Antibody | anti-TNFSF7 antibody

Polyclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 7 (TNFSF7)

Gene Names
CD70; CD27L; CD27-L; CD27LG; TNFSF7; TNLG8A
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Tumor Necrosis Factor Ligand Superfamily; Member 7 (TNFSF7); Polyclonal Antibody; Polyclonal Antibody to Tumor Necrosis Factor Ligand Superfamily; anti-TNFSF7 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-TNFSF7 antibody
WB; IHC; ICC; IP.
Application Notes
Western blotting: 0.5-2ug/mL;1:250-1000
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Organism Species
Mus musculus (Mouse))
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Human
Immunogen
Recombinant TNFSF7 (Leu64~Trp192 (Accession # O55237)) expressed in E.coli
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Ovary tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded Ovary tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
970
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
CD70 antigen isoform 1
NCBI Official Synonym Full Names
CD70 molecule
NCBI Official Symbol
CD70
NCBI Official Synonym Symbols
CD27L; CD27-L; CD27LG; TNFSF7; TNLG8A
NCBI Protein Information
CD70 antigen
UniProt Protein Name
CD70 antigen
UniProt Gene Name
CD70
UniProt Synonym Gene Names
CD27L; CD27LG; TNFSF7; CD27-L

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis. [provided by RefSeq, Jul 2008]

Uniprot Description

CD70: Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. Belongs to the tumor necrosis factor family.

Protein type: Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: integral component of plasma membrane; plasma membrane

Molecular Function: cytokine activity; protease binding; protein binding; receptor binding; tumor necrosis factor receptor binding

Biological Process: cell proliferation; cell-cell signaling; immune response; signal transduction; tumor necrosis factor-mediated signaling pathway

Research Articles on TNFSF7

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Product Notes

The TNFSF7 cd70 (Catalog #AAA2005722) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 7 (TNFSF7) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Tumor Necrosis Factor Ligand Superfamily, Member 7 (TNFSF7) can be used in a range of immunoassay formats including, but not limited to, WB; IHC; ICC; IP. Western blotting: 0.5-2ug/mL;1:250-1000 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the TNFSF7 cd70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor Ligand Superfamily, Member 7 (TNFSF7), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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