Angiogenin (ANG) Recombinant Protein | ANG recombinant protein

Recombinant Angiogenin (ANG)

Cat. Num. AAA2009655

• Gene Names: Ang; Ang1; Rnase5; Rnase5a; AI385586
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Angiogenin (ANG); Recombinant Angiogenin (ANG); ANG recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-QDDSRY TKFLTQHHDA KPKGRDDRYC ERMMKRRSLT SPCKDVNTFI HGNKSNIKAI CGANGSPYRE NLRMSKSPFQ VTTCKHTGGS PRPPCQYRAS AGFRHVVIAC ENGLPVHFDE SFFSL
• Sequence Length: 145

• Applicable Applications for ANG recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Gln25~Leu145 (Accession # P21570) with two N-terminal Tags, His-tag and GST-tag
• Subcellular Location: Secreted
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 8.2
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 239835771
• NCBI GeneID: 11727
• NCBI Accession #: NP_001155203.1
• NCBI GenBank Nucleotide #: NM_001161731.2
• UniProt Accession #: P21570
• Molecular Weight: 45.7kDa
• NCBI Official Full Name: angiogenin
• NCBI Official Synonym Full Names: angiogenin, ribonuclease, RNase A family, 5
• NCBI Official Symbol: Ang
• NCBI Official Synonym Symbols: Ang1; Rnase5; Rnase5a; AI385586
• NCBI Protein Information: angiogenin; RNase 5; angiogenin-1; ribonuclease 5; angiogenin, ribonuclease A family, member 1
• UniProt Protein Name: Angiogenin
• Protein Family: Angiogenin
• UniProt Gene Name: Ang
• UniProt Synonym Gene Names: Ang1; Rnase5; Rnase5a; RNase 5
• UniProt Entry Name: ANGI_MOUSE

NCBI Description

This gene encodes a member of the pancreatic ribonuclease A superfamily and is a potent inducer of neovascularization. The encoded protein is a secreted multifunctional tRNA-specific ribonuclease that promotes angiogenesis in response to angiogenetic stimuli such as hypoxia, mediates stress-induced translational repression by cleaving cellular tRNAs, stimulates cell proliferation by mediating rRNA transcription in prostate cancer cells, and is involved in neurite pathfinding. This gene resides in a cluster of highly related genes. It shares dual promoters and 5' exons with the ribonuclease, RNase A family 4 gene. Two alternatively spliced variants, with different 5' exons but the same coding exon, have been identified. Multiple pseudogenes have been found for this gene. [provided by RefSeq, Jun 2009]

Uniprot Description

ANG: May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9). ALS is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord. ALS is characterized by muscular weakness and atrophy. Belongs to the pancreatic ribonuclease family.

Protein type: Secreted, signal peptide; Nucleolus; Ribonuclease; Secreted; RNA-binding; Motility/polarity/chemotaxis; Cell cycle regulation; EC 3.1.27.-; Extracellular matrix

Cellular Component: proteinaceous extracellular matrix; extracellular space; angiogenin-PRI complex; growth cone; cell soma; extracellular region; basement membrane; basal lamina; nucleus

Molecular Function: heparin binding; copper ion binding; nuclease activity; nucleic acid binding; DNA binding; hydrolase activity; RNA binding; endonuclease activity; actin binding; peptide binding; receptor binding; ribonuclease activity

Biological Process: cell migration; central nervous system development; negative regulation of smooth muscle cell proliferation; diacylglycerol biosynthetic process; activation of protein kinase B; multicellular organismal development; response to hormone stimulus; activation of phospholipase A2; rRNA transcription; positive regulation of protein secretion; phospholipase C activation; negative regulation of translation; response to hypoxia; positive regulation of endothelial cell proliferation; angiogenesis; cell differentiation; positive regulation of phosphorylation