Rabbit MS4A6A / MS4A Polyclonal Antibody | anti-MS4A6A antibody

Rabbit Polyclonal to Human MS4A6A / MS4A

Cat. Num. AAA248560

• Gene Names: MS4A6A; CDA01; MS4A6; 4SPAN3; CD20L3; MST090; MSTP090; 4SPAN3.2
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Peptide Column Affinity Chromatography
• Synonyms: MS4A6A / MS4A; Polyclonal Antibody; Rabbit Polyclonal to Human MS4A6A / MS4A; Anti-MS4A6A / MS4A Antibody (N-Terminus) IHC-plus; MS4A6A; 4SPAN3.2; 4SPAN3; CD20-like precusor; CD20L3; CDA01; HAIRB-iso; MS4A; MST090; MS4A6; MS4A6A-polymorph; MSTP090; CD20 antigen-like 3; Human MS4A6A; anti-MS4A6A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human MS4A6A. At least three isoforms of MS4A6A are known to exist; this antibody will detect the two longer isoforms. MS4A6A antibody is predicted to not cross-react with MS4A family members.
• Purity/Purification: Peptide Column Affinity Chromatography
• Form/Format: PBS, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 225

• Applicable Applications for anti-MS4A6A antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (20 ug/ml), IHC-P (5 ug/ml), WB (1 - 2 ug/ml)
• Immunnogen Species: Human
• Immunogen Description: MS4A6A/ MS4A antibody was raised against a 19 amino acid synthetic peptide near the N-Terminus of human MS4S6S. The immunogen is located within the first 50amino acids of MS4A6A.
• Immunogen Type: Synthetic peptide
• Antigen Modification: N-Terminus
• Usage: MS4A6A antibody can be used for detection of MS4A6A by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4°C for up to 3 months. ; Store at -20°C for up to 1 year.; Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-MS4A6A / MS4A antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of MS4A6A in rat brain tissue with MS4A6A antibody at 20 µg/mL.)

Western Blot (WB)

(Western blot analysis of MS4A6A in 293 cell lysate with MS4A6A antibody at (A) 1 and (B) 2 ug/ml.)

Product Categories/Family for anti-MS4A6A antibody

Family: MS4A

NCBI and Uniprot Product Information

• NCBI GI #: 23238234
• NCBI GeneID: 64231
• NCBI Accession #: NP_071744.2
• NCBI GenBank Nucleotide #: NM_022349.3
• UniProt Accession #: Q9H2W1; Q7Z4E8; Q8TBV7; Q8TEZ4; Q8TEZ5; Q96PG6; Q9H2L1; Q9H2N3; Q9H3V1; Q9HC76; A8K755; F8W9K1
• NCBI Official Full Name: membrane-spanning 4-domains subfamily A member 6A isoform 2
• NCBI Official Synonym Full Names: membrane-spanning 4-domains, subfamily A, member 6A
• NCBI Official Symbol: MS4A6A
• NCBI Official Synonym Symbols: CDA01; MS4A6; 4SPAN3; CD20L3; MST090; MSTP090; 4SPAN3.2
• NCBI Protein Information: membrane-spanning 4-domains subfamily A member 6A; HAIRB-iso; MS4A6A-polymorph; CD20-like precusor; CD20 antigen-like 3; four-span transmembrane protein 3; four-span transmembrane protein 3.1; four-span transmembrane protein 3.2; membrane-spanning 4-domains, subfamily A, member 6A, isoform 2
• UniProt Protein Name: Membrane-spanning 4-domains subfamily A member 6A
• Protein Family: Membrane-spanning 4-domains subfamily
• UniProt Gene Name: MS4A6A
• UniProt Synonym Gene Names: 4SPAN3; CD20L3; MS4A6
• UniProt Entry Name: M4A6A_HUMAN

NCBI Description

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. The gene encoding this protein is localized to 11q12.1, among a cluster of family members. Alternative splicing of this gene results in several transcript variants that encode different protein isoforms. [provided by RefSeq, Oct 2011]

Uniprot Description

MS4A6A: May be involved in signal transduction as a component of a multimeric receptor complex. Belongs to the MS4A family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cell surface

Chromosomal Location of Human Ortholog: 11q12.1

Cellular Component: integral to membrane