Rabbit TUC-4 Protein Polyclonal Antibody

TUC-4 Protein (TOAD, Turned On After Division, Ulip/CRMP-4, DRP-3, Dihydropyrimidinase-like 3)

Cat. Num. AAA612306

• Reactivity: Feline, Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
• Purity: Serum; Serum
• Synonyms: TUC-4 Protein; Polyclonal Antibody; TUC-4 Protein (TOAD; Turned On After Division; Ulip/CRMP-4; DRP-3; Dihydropyrimidinase-like 3); Anti -TUC-4 Protein (TOAD; anti-TUC-4 Protein antibody

• Host: Rabbit
• Reactivity: Feline, Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TUC-4 protein (ULIP1/CRMP4). Reported to label early postmitotic neurons in prenatal rat brain. Not reactive with the proteins TUC-1 (ULIP3/CRMP1), TUC-2 (ULIP2/CRMP2) or TUC-3 (ULIP4/CRMP3). Does not label neural progenitor or radial glia. Species Crossreactivity: Rat, mouse, feline and monkey.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid, 0.2% sodium azide.

• Applicable Applications for anti-TUC-4 Protein antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.; Dilution: Western Blot: 1:2500-1:25,000 using akaline phosphatase; Immunohistochemistry: 1:1000-1:5000 using PFA (DAB detection).; Immunocytochemistry: 1:1000-5000 (DAB detection).
• Immunogen: Synthetic peptide corresponding to amino acids 499 to 511 of TUC-4a and amino acids 612-624 of TUC-4b (YDGPVFDLTTTPK)
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemical staining of TUC04 in the neuronal growth cone.)

Related Product Information for anti-TUC-4 Protein antibody

TUC (TOAD [Turned On After Division]/ Ulip/CRMP) is a family of proteins that have emerged as strong candidates for a role in neuronal growth cone signaling. The TUC-4 family members reach their highest expression levels in early post-mitotic neurons during their peak periods of axonal growth. TUC-4 is not expressed in neural progenitors or adult neurons (with minor exception). Data suggests that TUC-4 plays a role in axonal outgrowth, pathfinding and possibly in the process that permits growing axons to choose proper routes and targets (4). The TUC-4 protein is expressed primarily within the cytoplasm of postmitotic neurons as they begin their migration out of the ventricular zone into the developing cortical plate (2). TUC-4 is expressed in neuronal cell bodies, neurites, and along the most distal regions of neuronal growth cones, including the lamellipodia and filopodia (3). Expression of TUC-4 is observed by day E12 in rats coincident with the expression of the early neuronal marker class III beta-tubulin (4). TUC-4 expression is rarely observed in neuronal cells expressing the mitotic marker PCNA (2). The expression of TUC-4 has been shown to be upregulated in response to NGF in rats (3) and in response to retinoic acid in a human neuroblastoma cell line (SMS-KCNR).

Product Categories/Family for anti-TUC-4 Protein antibody

Antibodies; Abs to Proteins