Rabbit TUBULIN Polyclonal Antibody | anti-tubulin antibody

RABBIT ANTI TUBULIN

Cat. Num. AAA221198

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Synonyms: TUBULIN; Polyclonal Antibody; RABBIT ANTI TUBULIN; anti-tubulin antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item detects tubulin, a protein which is the major constituent of microtubules. Tubulin is a dimer of alpha and beta chains, which binds two molecules of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. There are at least six isotypes of both alpha- and beta- tubulin in human cells, which are distinguished by slightly different amino acid sequences and encoded by a large, multigene family that has been highly conserved throughout evolution. ; Although the most important functions of microtubules in proliferative cells are through their actions as components of the mitotic spindle, they are also involved in many other essential functions throughout the cell cycle of both malignant and nonmalignant cells. Antimicrotubule agents including Vinca alkaloids and taxanes may disrupt many of these essential functions.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 5.0mg/ml (varies by lot)
• Sequence Length: 423

• Applicable Applications for anti-tubulin antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: Minimum Dilution: 1/50; Maximum Dilution: 1/500;; Western Blotting: Minimum Dilution: 1/250; Maximum Dilution: 1/1000
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Immunogen: Purified porcine brain tubulin; Buffer Solution
• Antiserum Preparation: Antisera to tubulin were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Pig
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-tubulin antibody

MBS221198 detects tubulin, a protein which is the major constituent of microtubules. Tubulin is a dimer of alpha and beta chains, which binds two molecules of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. There are at least six isotypes of both alpha- and beta- tubulin in human cells, which are distinguished by slightly different amino acid sequences and encoded by a large, multigene family that has been highly conserved throughout evolution. Although the most important functions of microtubules in proliferative cells are through their actions as components of the mitotic spindle, they are also involved in many other essential functions throughout the cell cycle of both malignant and nonmalignant cells. Antimicrotubule agents including Vinca alkaloids and taxanes may disrupt many of these essential functions.

NCBI and Uniprot Product Information

• NCBI GI #: 687675227
• Molecular Weight: 46,955 Da
• NCBI Official Full Name: tubulin, partial
• UniProt Protein Name: Tubulin
• Protein Family: Tubulin
• UniProt Gene Name: tubulin
• UniProt Entry Name: A0A090I464_9EUKA

Uniprot Description

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.