Western Blot (WB)
(Western blot analysis of TUB 1 expression in COLO320 whole cell lysates (lane 1) and MCF-7 whole cell lysates (lane 2). TUB 1 at 62KD was detected using rabbit anti- TUB 1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Rabbit anti-Human TUB 1 Polyclonal Antibody | anti-TUB antibody
Anti-TUB 1 Antibody
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Other applications have not been tested.
Western Blot (WB)
(Western blot analysis of TUB 1 expression in COLO320 whole cell lysates (lane 1) and MCF-7 whole cell lysates (lane 2). TUB 1 at 62KD was detected using rabbit anti- TUB 1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Western Blot (WB)
(Western blot analysis of TUB 1 expression in COLO320 whole cell lysates (lane 1) and MCF-7 whole cell lysates (lane 2). TUB 1 at 62KD was detected using rabbit anti- TUB 1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Background: Tubby protein homolog is a protein that in humans is encoded by the TUB gene. This gene encodes a member of the Tubby family of bipartite transcription factors. The encoded protein may play a role in obesity and sensorineural degradation. The crystal structure has been determined for a similar protein in mouse, and it functions as a membrane-bound transcription regulator that translocates to the nucleus in response to phosphoinositide hydrolysis. Two transcript variants encoding distinct isoforms have been identified for this gene.
2. Kapeller, R; Moriarty A; Strauss A; Stubdal H; Theriault K; Siebert E; Chickering T; Morgenstern J P; Tartaglia L A; Lillie J (Aug 1999). "Tyrosine phosphorylation of tub and its association with Src homology 2 domain-containing proteins implicate tub in intracellular signaling by insulin". J. Biol. Chem. UNITED STATES. 274 (35): 24980-6.
3. Kleyn PW, Fan W, Kovats SG, Lee JJ, Pulido JC, Wu Y, Berkemeier LR, Misumi DJ, Holmgren L, Charlat O, Woolf EA, Tayber O, Brody T, Shu P, Hawkins F, Kennedy B, Baldini L, Ebeling C, Alperin GD, Deeds J, Lakey ND, Culpepper J, Chen H, Glucksmann-Kuis MA, Carlson GA, Duyk GM, Moore KJ (Jun 1996). "Identification and characterization of the mouse obesity gene tubby: a member of a novel gene family". Cell. 85 (2): 281-90.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Tubby family of bipartite transcription factors. The encoded protein may play a role in obesity and sensorineural degradation. The crystal structure has been determined for a similar protein in mouse, and it functions as a membrane-bound transcription regulator that translocates to the nucleus in response to phosphoinositide hydrolysis. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
tubby: an effector protein for heterotrimeric G protein-coupled receptors. Binds phospholipid and is anchored to the plasma membrane through binding phosphatidylinositol-4,5-bisphosphate. Is released upon activation of phospholipase C. Translocates from the plasma membrane to the nucleus upon activation by the G-alpha(q)/(11) subclass of G proteins that are linked to receptors including the seratonin receptor 5-HT(2C). Can bind DNA in vitro and may contribute to the regulation of transcription in the nucleus. TUB can be phosphorylated by the insulin receptor and may participate in insulin receptor signaling. Does not have a cleavable signal peptide and is secreted by a non-conventional pathway. Highly expressed in the paraventricular nucleus of the hypothalamus and several other brain regions. May play a role in the hypothalamic regulation of body weight. Contributes to stimulation of phagocytosis of apoptotic retinal pigment epithelium (RPE) cells and macrophages. Belongs to the TUB family. Mutations in the tubby promote maturity-onset obesity in mice. Interacts with GNAQ. Interacts with TULP1. Two isoforms of the human protein are produced through alternative splicing.
Protein type: Lipid-binding; Membrane protein, peripheral
Chromosomal Location of Human Ortholog: 11p15.4
Cellular Component: cilium; cytoplasm
Molecular Function: phosphoinositide binding; protein complex binding
Biological Process: positive regulation of phagocytosis
Disease: Retinal Dystrophy And Obesity
Research Articles on TUB
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Product Notes
The TUB tub (Catalog #AAA178674) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TUB 1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TUB 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the TUB tub for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TUB 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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