TTR / Transthyretin Polyclonal Antibody | anti-TTR antibody

Goat Polyclonal to Human TTR / Transthyretin

Cat. Num. AAA241997

• Gene Names: TTR; CTS; CTS1; PALB; TBPA; HEL111; HsT2651
• Reactivity: Human, Monkey; Predicted Reactivity: Bovine, Dog, Pig, Rabbit, Sheep
• Applications: ELISA, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: TTR / Transthyretin; Polyclonal Antibody; Goat Polyclonal to Human TTR / Transthyretin; TTR; ATTR; CTS1; CTS; HsT2651; PALB; Prealbumin; TBPA; Transthyretin; Carpal tunnel syndrome 1; amyloidosis type I; Thyroxine-binding prealbumin; anti-TTR antibody

• Host: TTR/Transthyretin antibody was produced in Goat.
• Reactivity: Human, Monkey; Predicted Reactivity: Bovine, Dog, Pig, Rabbit, Sheep
• Clonality: Polyclonal
• Specificity: Human TTR / Transthyretin.
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 147

• Applicable Applications for anti-TTR antibody: IHC; IHC - Paraffin (10 ug/ml); ELISA (5 - 10 ug/ml); Peptide Enzyme-Linked Immunosorbent Assay (1:1000); ; Optimal dilution to be determined by the researcher).
• Target: TTR/Transthyretin
• Immunogen Description: Synthetic peptide C-YKVEIDTKSYWK from an internal region of human TTR / Transthyretin (NP_000362.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%).
• Immunogen Species: TTR/Transthyretin antibody was raised against Human.
• Epitope: AA89-100
• Antigen Type: Synthetic peptide
• Immunogen: TTR / Transthyretin antibody was raised against synthetic peptide C-YKVEIDTKSYWK from an internal region of human TTR / Transthyretin (NP_000362.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%); Sheep, Elephant, Panda, Dog, Bovine, Rabbit, Pig (92%); Mouse, Rat, Hamster, Bat, Horse, Platypus (83%).
• Antigen Modification: Internal
• Usage Summary: Immunohistochemistry: MBS241997 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat inudced antigen retrieval in pH6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS241997 was determined to be 10 ug/ml
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
• Preparation and Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-TTR / Transthyretin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Related Product Information for anti-TTR antibody

Prealbumin, also known as transethrytin (TTR) is a carrier protein; it transports thyroid hormones in the plasma and cerebrospinal fluid, and also transports retinol (vitamin A) in the plasma. The protein consists of a tetramer of identical subunits. More than 80 different mutations in this gene have been reported; most mutations are related to amyloid deposition, affecting predominantly peripheral nerve and/or the heart, and a small portion of the gene mutations is non-amyloidogenic.

NCBI and Uniprot Product Information

• NCBI GI #: 4507725
• NCBI GeneID: 7276
• NCBI Accession #: NP_000362.1
• NCBI GenBank Nucleotide #: NM_000371.3
• UniProt Accession #: P02766; Q549C7; Q6IB96; Q9UBZ6; Q9UCM9
• NCBI Official Full Name: transthyretin
• NCBI Official Synonym Full Names: transthyretin
• NCBI Official Symbol: TTR
• NCBI Official Synonym Symbols: CTS; CTS1; PALB; TBPA; HEL111; HsT2651
• NCBI Protein Information: transthyretin; ATTR; carpal tunnel syndrome 1; thyroxine-binding prealbumin; epididymis luminal protein 111; prealbumin, amyloidosis type I
• UniProt Protein Name: Transthyretin
• Protein Family: Transthyretin
• UniProt Gene Name: TTR
• UniProt Synonym Gene Names: PALB
• UniProt Entry Name: TTHY_HUMAN

NCBI Description

This gene encodes transthyretin, one of the three prealbumins including alpha-1-antitrypsin, transthyretin and orosomucoid. Transthyretin is a carrier protein; it transports thyroid hormones in the plasma and cerebrospinal fluid, and also transports retinol (vitamin A) in the plasma. The protein consists of a tetramer of identical subunits. More than 80 different mutations in this gene have been reported; most mutations are related to amyloid deposition, affecting predominantly peripheral nerve and/or the heart, and a small portion of the gene mutations is non-amyloidogenic. The diseases caused by mutations include amyloidotic polyneuropathy, euthyroid hyperthyroxinaemia, amyloidotic vitreous opacities, cardiomyopathy, oculoleptomeningeal amyloidosis, meningocerebrovascular amyloidosis, carpal tunnel syndrome, etc. [provided by RefSeq, Jan 2009]

Uniprot Description

TTR: Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain. Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR). A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor. Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE). It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities. Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1). It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis. Belongs to the transthyretin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 18q12.1

Cellular Component: extracellular space; protein complex; cytoplasm; extracellular region

Molecular Function: identical protein binding; protein binding; protein heterodimerization activity; hormone activity

Biological Process: phototransduction, visible light; extracellular matrix organization and biogenesis; retinol metabolic process; transport; retinoid metabolic process

Disease: Hyperthyroxinemia, Dystransthyretinemic; Carpal Tunnel Syndrome; Amyloidosis, Hereditary, Transthyretin-related