Rabbit anti-Human AGAP3 Polyclonal Antibody | anti-AGAP3 antibody

AGAP3, NT (AGAP3, CENTG3, Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3, CRAM-associated GTPase, Centaurin-gamma-3, MR1-interacting protein) (PE)

Cat. Num. AAA6272156

• Gene Names: AGAP3; CRAG; AGAP-3; CENTG3; MRIP-1; cnt-g3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AGAP3; Polyclonal Antibody; NT (AGAP3; CENTG3; Arf-GAP with GTPase; ANK repeat and PH domain-containing protein 3; CRAM-associated GTPase; Centaurin-gamma-3; MR1-interacting protein) (PE); MR1-interacting protein; anti-AGAP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-AGAP3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: AGAP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-217 amino acids from the N-terminal region of human AGAP3.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(AGAP3 Antibody (N-term) western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the AGAP3 antibody detected the AGAP3 protein (arrow).)

Related Product Information for anti-AGAP3 antibody

GTPase-activating protein for the ADP ribosylation factor family (Potential). GTPase which may be involved in the degradation of expanded polyglutamine proteins through the ubiquitin-proteasome pathway.

Product Categories/Family for anti-AGAP3 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006). Qin, Q., et al. J. Cell Biol. 172(4):497-504(2006). Meurer, S., et al. J. Biol. Chem. 279(47):49346-49354(2004). Surks, H.K., et al. J. Biol. Chem. 278(51):51484-51493(2003). Xia, C., et al. Mol. Cell. Biol. 23(7):2476-2488(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 110227617
• NCBI GeneID: 116988
• NCBI Accession #: NP_001036000.1; NP_114152.3
• NCBI GenBank Nucleotide #: NM_001042535.3
• UniProt Accession #: Q96P47; Q59EN0; Q96RK3; B3KNZ8; E9PAL8
• Molecular Weight: 42,517 Da
• NCBI Official Full Name: arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 isoform b
• NCBI Official Synonym Full Names: ArfGAP with GTPase domain, ankyrin repeat and PH domain 3
• NCBI Official Symbol: AGAP3
• NCBI Official Synonym Symbols: CRAG; AGAP-3; CENTG3; MRIP-1; cnt-g3
• NCBI Protein Information: arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3
• UniProt Protein Name: Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3
• Protein Family: Arf-GAP with GTPase, ANK repeat and PH domain-containing protein
• UniProt Gene Name: AGAP3
• UniProt Synonym Gene Names: CENTG3; AGAP-3; CRAG; Cnt-g3; MRIP-1
• UniProt Entry Name: AGAP3_HUMAN

NCBI Description

This gene encodes an essential component of the N-methyl-D-aspartate (NMDA) receptor signaling complex which mediates long-term potentiation in synapses by linking activation of NMDA receptor to alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor trafficking. The encoded protein contains an N-terminal GTPase-like domain, a pleckstrin homology domain, an ArfGAP domain and several C-terminal ankryn repeat domains. [provided by RefSeq, Apr 2017]

Uniprot Description

CENTG3: a member of the centaurin family of ADP-ribosylation factor directed (ARF) GTPase-activating proteins (GAPs). Protective against the pathogenesis of polyglutamine diseases. Mediates the nuclear translocation of the CENTG3-polyQ complexes and the formation of promyelocytic leukemia (PML) protein-associated nuclear bodies in reactive oxygen species (ROS) signaling. Promotes the degradation of polyQ proteins at PML nuclear bodies through the ubiquitin-proteasome pathway. Knockdown by small interfering RNA in neuronal cells consistently blocked the nuclear translocation of polyQ and enhanced polyQ-mediated cell death. Three alternatively-spliced isoforms have been described.

Protein type: GAPs; GAPs, ARF

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: membrane; cytoplasm; nucleus

Molecular Function: GTPase activity; GTP binding; zinc ion binding; polyubiquitin binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein import into nucleus, translocation; small GTPase mediated signal transduction; positive regulation of GTPase activity