Rabbit anti-Human, Mouse TTBK1 Polyclonal Antibody | anti-TTBK1 antibody

TTBK1, NT (TTBK1, BDTK, KIAA1855, Tau-tubulin kinase 1, Brain-derived tau kinase) (Azide free) (HRP)

Cat. Num. AAA6359819

• Gene Names: TTBK1; BDTK
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TTBK1; Polyclonal Antibody; NT (TTBK1; BDTK; KIAA1855; Tau-tubulin kinase 1; Brain-derived tau kinase) (Azide free) (HRP); Brain-derived tau kinase; anti-TTBK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TTBK1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TTBK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 240-270 from the N-terminal region of human TTBK1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TTBK1 Antibody (N-term) in mouse testis tissue lysates (35ug/lane). TTBK1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(TTBK1 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TTBK1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-TTBK1 antibody

TTBK1 is Serine/threonine kinase which is able to phosphorylate TAU on serine, threonine and tyrosine residues. Induces aggregation of TAU.

Product Categories/Family for anti-TTBK1 antibody

Antibodies; Protein Kinases

References

Sato, S., et al. J. Neurosci. 28(53):14511-14521(2008). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007). Mungall, A.J., et al. Nature 425(6960):805-811(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 97203020
• NCBI GeneID: 84630
• UniProt Accession #: Q5TCY1; Q2L6C6; Q6ZNH0; Q8N444; Q96JH2; A2A2U5
• Molecular Weight: 69,084 Da
• NCBI Official Full Name: Tau-tubulin kinase 1
• NCBI Official Synonym Full Names: tau tubulin kinase 1
• NCBI Official Symbol: TTBK1
• NCBI Official Synonym Symbols: BDTK
• NCBI Protein Information: tau-tubulin kinase 1; brain-derived tau kinase
• UniProt Protein Name: Tau-tubulin kinase 1
• Protein Family: Tau-tubulin kinase
• UniProt Gene Name: TTBK1
• UniProt Synonym Gene Names: BDTK; KIAA1855
• UniProt Entry Name: TTBK1_HUMAN

Uniprot Description

TTBK1: Serine/threonine kinase which is able to phosphorylate TAU on serine, threonine and tyrosine residues. Induces aggregation of TAU. Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, CK1; CK1 group; TTBK family

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; ATP binding

Biological Process: regulation of cell shape; peptidyl-serine phosphorylation; substantia nigra development