Immunofluorescence (IF)
(Fluorescent image of Hela cell stained with CBFA2T2 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CBFA2T2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).CBFA2T2 immunoreactivity is localized to Nucleus significantly. )
Rabbit anti-Human CBFA2T2 Polyclonal Antibody | anti-CBFA2T2 antibody
CBFA2T2, NT (CBFA2T2, EHT, MTGR1, Protein CBFA2T2, ETO homologous on chromosome 20, MTG8-like protein, MTG8-related protein 1, Myeloid translocation-related protein 1, p85) (PE)
Gene Names
CBFA2T2; EHT; p85; MTGR1; ZMYND3
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CBFA2T2; Polyclonal Antibody; NT (CBFA2T2; EHT; MTGR1; Protein CBFA2T2; ETO homologous on chromosome 20; MTG8-like protein; MTG8-related protein 1; Myeloid translocation-related protein 1; p85) (PE); p85; anti-CBFA2T2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-CBFA2T2 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
CBFA2T2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CBFA2T2.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunofluorescence (IF)
(Fluorescent image of Hela cell stained with CBFA2T2 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CBFA2T2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).CBFA2T2 immunoreactivity is localized to Nucleus significantly. )
Immunofluorescence (IF)
(Fluorescent image of Hela cell stained with CBFA2T2 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CBFA2T2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).CBFA2T2 immunoreactivity is localized to Nucleus significantly. )
Western Blot (WB)
(CBFA2T2 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CBFA2T2 antibody detected the CBFA2T2 protein (arrow).)
Western Blot (WB)
(CBFA2T2 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CBFA2T2 antibody detected the CBFA2T2 protein (arrow).)
Related Product Information for anti-CBFA2T2 antibody
In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described.
Product Categories/Family for anti-CBFA2T2 antibody
References
Guastadisegni, M.C., et al. Leukemia 24(8):1516-1519(2010). Ossovskaya, V.S., et al. J. Neurosci. Methods 177(2):322-333(2009). Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009). Olsson, A., et al. Biochim. Biophys. Acta 1779(10):590-598(2008). Kumar, R., et al. Mol. Cancer Res. 4(9):655-665(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
67
NCBI Official Full Name
protein CBFA2T2 isoform MTGR1c
NCBI Official Synonym Full Names
core-binding factor, runt domain, alpha subunit 2; translocated to, 2
NCBI Official Symbol
CBFA2T2
NCBI Official Synonym Symbols
EHT; p85; MTGR1; ZMYND3
NCBI Protein Information
protein CBFA2T2; MTG8-like protein; MTG8-related protein 1; ETO homolog on chromosome 20; ETO homologous on chromosome 20; myeloid translocation-related protein 1; myeloid translocation gene-related protein 1
UniProt Protein Name
Protein CBFA2T2
Protein Family
UniProt Gene Name
CBFA2T2
UniProt Synonym Gene Names
EHT; MTGR1
UniProt Entry Name
MTG8R_HUMAN
NCBI Description
In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described. [provided by RefSeq, Jul 2008]
Research Articles on CBFA2T2
Similar Products
Product Notes
The CBFA2T2 cbfa2t2 (Catalog #AAA6281111) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CBFA2T2, NT (CBFA2T2, EHT, MTGR1, Protein CBFA2T2, ETO homologous on chromosome 20, MTG8-like protein, MTG8-related protein 1, Myeloid translocation-related protein 1, p85) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CBFA2T2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CBFA2T2 cbfa2t2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CBFA2T2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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