Rabbit TSPO/PBR Polyclonal Antibody | anti-TSPO/PBR antibody

TSPO/PBR Polyclonal Antibody

Cat. Num. AAA3015175

• Gene Names: TSPO; DBI; IBP; MBR; PBR; PBS; BPBS; BZRP; PKBS; PTBR; mDRC; pk18
• Reactivity: Human, Mouse, Rat, Dog, Pig
• Applications: Immunohistochemistry
• Purity: affinity purified by Protein A
• Synonyms: TSPO/PBR; Polyclonal Antibody; TSPO/PBR Polyclonal Antibody; BPBS; BZRP; DBI; IBP; Isoquinoline carboxamide-binding protein; MBR; mDRC; Mitochondrial benzodiazepine receptor; PBR; PBS; Peripheral benzodiazepine receptor; Peripheral benzodiazepine receptor-related protein; Peripheral type benzodiazepine receptor; pk18; PKBS; PTBR; Ptbzr; PTBZR02; RATPTBZR02; translocator protein (18kDa); Translocator protein; TSPO; Tspo1; Benzodiazapine receptor (peripheral); Benzodiazepine peripheral binding site; anti-TSPO/PBR antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Dog, Pig
• Clonality: Polyclonal
• Specificity: TSPO/PBR Polyclonal Antibody detects endogenous levels of TSPO/PBR protein.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-TSPO/PBR antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:100-500
• Immunogen: KLH conjugated synthetic peptide derived from human PBR
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PBR) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PBR) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C)

Related Product Information for anti-TSPO/PBR antibody

PBR (Peripheral type Benzodiazepine Receptor) is a ubiquitous protein (18 kDa) found in most steroidogenic tissues. It is located primarily in the outer mitochondrial membrane of steroid producing tissues and is found in association with a 34 kDa voltage dependent anion channel protein (VDAC). PBR interacts with some benzodiazepines and has different affinities than its endogenous counterpart. PBR appears to be a key factor in the transport of cholesterol from the outer to the inner mitochondrial membrane to permit the initiation of steroid hormone synthesis. It is thought to be part of the mitochondrial permeability transition pore (PT) and to be involved in apoptosis. It is speculated that patients with congenital lipoid adrenal hyperplasia, who cannot make any steroids, might have a genetic lesion in BZRP. A short form, PBRS is also expressed in the same tissues, but at a level about ten times that of PBR.

NCBI and Uniprot Product Information

• NCBI GI #: 254789861
• NCBI GeneID: 706
• UniProt Accession #: B1AH88; Q13849; Q6IAZ7; P30536
• Molecular Weight: 18,828 Da
• NCBI Official Full Name: Putative peripheral benzodiazepine receptor-related protein
• NCBI Official Synonym Full Names: translocator protein
• NCBI Official Symbol: TSPO
• NCBI Official Synonym Symbols: DBI; IBP; MBR; PBR; PBS; BPBS; BZRP; PKBS; PTBR; mDRC; pk18
• NCBI Protein Information: translocator protein
• UniProt Protein Name: Putative peripheral benzodiazepine receptor-related protein
• UniProt Gene Name: TSPO
• UniProt Synonym Gene Names: PBRS

NCBI Description

Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternatively spliced transcript variants have been reported; one of the variants lacks an internal exon and is considered non-coding, and the other variants encode the same protein. [provided by RefSeq, Feb 2012]