Rabbit TSLP Polyclonal Antibody | anti-TSLP antibody

TSLP Polyclonal Antibody

Cat. Num. AAA3011447

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TSLP; Polyclonal Antibody; TSLP Polyclonal Antibody; Thymic stromal lymphopoietin; anti-TSLP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TSLP polyclonal antibody detects endogenous levels of TSLP protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 63

• Applicable Applications for anti-TSLP antibody: Western Blot (WB)
• Application Notes: WB: 1:1000-1:3000
• Immunogen: Recombinant full length Human TSLP.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using TSLP antibody at 1:3000 dilution.)

Related Product Information for anti-TSLP antibody

Thymic stromal lymphopoietin (TSLP) is a novel member of the hemopoietic cytokine family that promotes the development of B cells and shares overlapping activity with IL-7. The gene encoding murine TSLP maps to chromosome 18 and its human homologue is expressed in several tissues, including heart, liver and prostate. TSLP mediates its function by binding to a receptor complex. TSLP first binds with low affinity to a TSLP-specific chain designated TSLPR, and then forms a high affinity complex with the IL-7Ralpha subunit, which explains the overlapping biological properties between TSLP and IL-7. Both TSLP and IL-7 induce phosphorylation of the transcription factor Stat5, but unlike IL-7, TSLP-mediated signaling does not activate the JAKs. TSLP prevents apoptosis and stimulates the proliferation of myeloid cells, which is supported by the coexpression of TSLPR and IL-7RRalpha on monocytes and dendritic cells.

NCBI and Uniprot Product Information

• NCBI GI #: 190886449
• NCBI GeneID: 85480
• NCBI Accession #: NP_612561.2
• NCBI GenBank Nucleotide #: NP_612561.2
• UniProt Accession #: Q969D9
• Molecular Weight: ~ 18kDa
• NCBI Official Full Name: thymic stromal lymphopoietin isoform 2
• NCBI Official Synonym Full Names: thymic stromal lymphopoietin
• NCBI Official Symbol: TSLP
• NCBI Protein Information: thymic stromal lymphopoietin
• UniProt Protein Name: Thymic stromal lymphopoietin
• Protein Family: Thymic stromal lymphopoietin
• UniProt Gene Name: TSLP
• UniProt Entry Name: TSLP_HUMAN

NCBI Description

This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

TSLP: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q22.1

Cellular Component: extracellular space

Molecular Function: cytokine activity