TSC22D1 recombinant protein

TSC22D1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121217

• Gene Names: TSC22D1; Ptg-2; TSC22; TGFB1I4
• Purity: >92% as determined by SDS-PAGE
• Synonyms: TSC22D1; TSC22D1 Protein; Human; Recombinant (His Tag); Human TSC22D1 Protein (His Tag); Ptg-2 Protein; TGFB1I4 Protein; TSC22 Protein; TSC22 domain family; member 1; TSC22D1 recombinant protein

• Host: E Coli
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala144

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human TSC22D1 isoform 2 (Q15714-2)(Met 1-Ala 144) was expressed, with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TSC22D1 recombinant protein

Background: TSC22 domain family, member 1 (TSC22D1) is one of the TGF-beta-stimulated clone-22 (TSC-22). TSC-22 was reported to be a differentiation-inducing factor that negatively regulates the growth of salivary gland cancer cells. TSC22D1, which encodes transforming growth factor beta-stimulated clone 22 (TSC-22), is thought to be a tumor suppressor because its expression is lost in many glioblastoma, salivary gland, and prostate cancers. TSC-22 is the founding member of the TSC-22/DIP/Bun family of leucine zipper transcription factors. TSC-22 may play an important role in maintaining the differentiated phenotype in salivary gland tumors, and may be a possible target of leukemia therapy. TSC22D1 forms homodimers via its conserved leucine zipper domain and heterodimerizes with TSC22D4. TSC22D1 has transcriptional repressor activity.

References

Doi Y, et al. (2008) Expression and cellular localization of TSC-22 in normal salivary glands and salivary gland tumors: implications for tumor cell differentiation. Oncol Rep. 19(3): 609-16.Wu X, et al. (2008) The Drosophila homolog of human tumor suppressor TSC-22 promotes cellular growth, proliferation, and survival. Proc Natl Acad Sci U S A. 105(14): 5414-9.Lu Y, et al. (2007) Identification of TSC-22 as a potential tumor suppressor that is upregulated by Flt3-D835V but not Flt3-ITD. Leukemia. 21(11): 2246-57.

NCBI and Uniprot Product Information

• NCBI GI #: 345090986
• NCBI GeneID: 8848
• NCBI Accession #: NP_001230728.1
• NCBI GenBank Nucleotide #: NM_001243799.1
• UniProt Accession #: Q15714; O00666; Q6AHX5; Q6IBU1; Q8NCN1; Q96JS5; B3KRL7; B9EGI0
• Molecular Weight: 109,677 Da
• NCBI Official Full Name: TSC22 domain family protein 1 isoform 4
• NCBI Official Synonym Full Names: TSC22 domain family, member 1
• NCBI Official Symbol: TSC22D1
• NCBI Official Synonym Symbols: Ptg-2; TSC22; TGFB1I4
• NCBI Protein Information: TSC22 domain family protein 1; cerebral protein 2; regulatory protein TSC-22; TGFbeta-stimulated clone 22; TGFB-stimulated clone 22 homolog; transcriptional regulator TSC-22; transforming growth factor beta-stimulated protein TSC-22; transforming growth f
• UniProt Protein Name: TSC22 domain family protein 1
• Protein Family: TSC22 domain family protein
• UniProt Gene Name: TSC22D1
• UniProt Synonym Gene Names: KIAA1994; TGFB1I4; TSC22
• UniProt Entry Name: T22D1_HUMAN

NCBI Description

This gene encodes a member of the TSC22 domain family of leucine zipper transcription factors. The encoded protein is stimulated by transforming growth factor beta, and regulates the transcription of multiple genes including C-type natriuretic peptide. The encoded protein may play a critical role in tumor suppression through the induction of cancer cell apoptosis, and a single nucleotide polymorphism in the promoter of this gene has been associated with diabetic nephropathy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

TSC22D1: Transcriptional repressor. Acts on the C-type natriuretic peptide (CNP) promoter. Belongs to the TSC-22/Dip/Bun family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 13q14

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; positive regulation of apoptosis; positive regulation of cell proliferation; negative regulation of apoptosis