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Western Blot (WB) (Western Blot analysis of extracts of various cell lines, using TXN2 antibody.)

Rabbit Trx2 Polyclonal Antibody | anti-Trx2 antibody

Trx2 Polyclonal Antibody

Gene Names
TXN2; TXN; MTRX; TRX2; MT-TRX; COXPD29
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
Trx2; Polyclonal Antibody; Trx2 Polyclonal Antibody; Thioredoxin; mitochondrial; MTRX; Mt-Trx; Thioredoxin-2; Thioredoxin 2; Thioredoxin2; TXN2; TRX-2; anti-Trx2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Trx2 polyclonal antibody detects endogenous levels of Trx2 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
166
Applicable Applications for anti-Trx2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant full length Human Trx2.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using TXN2 antibody.)

Western Blot (WB) (Western Blot analysis of extracts of various cell lines, using TXN2 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using TXN2 antibody. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF) (Immunofluorescence analysis of U2OS cell using TXN2 antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-Trx2 antibody
Thioredoxin 2 (Trx-2) is a small redox protein that is localized to the mitochondria and is essential for cell viability, playing a crucial role in the scavenging of ROS in mitochondria and regulating the mitochondrial apoptosis signaling pathway. Trx reductases (TrxR1 and TrxR2) are ubiquitously expressed flavoproteins that catalyze the NADPH-dependent reduction of Trx as well as several other oxidized cellular components. Mammalian Trx reductases are a part of a selenium-containing pyridine nucleotide-disulphide oxidoreductase family, which has a conserved catalytic site of Cys-Val-Asn-Val-Gly-Cys. TrxR1 and TrxR2 are also involved in the prevention of oxidative stress. Inhibition of TrxR activity may provide for potential treatments of cancer, AIDS and other autoimmune diseases as well as bacterial infections and parasitic diseases.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 18kDa
NCBI Official Full Name
thioredoxin, mitochondrial
NCBI Official Synonym Full Names
thioredoxin 2
NCBI Official Symbol
TXN2
NCBI Official Synonym Symbols
TXN; MTRX; TRX2; MT-TRX; COXPD29
NCBI Protein Information
thioredoxin, mitochondrial
UniProt Protein Name
Thioredoxin, mitochondrial
Protein Family
UniProt Gene Name
TXN2
UniProt Synonym Gene Names
TRX2; MTRX; Mt-Trx
UniProt Entry Name
THIOM_HUMAN

NCBI Description

This nuclear gene encodes a mitochondrial member of the thioredoxin family, a group of small multifunctional redox-active proteins. The encoded protein may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

TXN2: Has an anti-apoptotic function and plays an important role in the regulation of mitochondrial membrane potential. Could be involved in the resistance to anti-tumor agents. Possesses a dithiol-reducing activity. Belongs to the thioredoxin family.

Protein type: Oxidoreductase

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cell soma; mitochondrion; mitochondrial matrix; dendrite; nucleolus

Molecular Function: peptide-methionine-(S)-S-oxide reductase activity; protein disulfide oxidoreductase activity; protein complex binding; peptide-methionine (R)-S-oxide reductase activity

Biological Process: response to drug; response to reactive oxygen species; cellular response to nutrient levels; cell redox homeostasis; response to glucose stimulus; response to hormone stimulus; response to hypoxia; response to axon injury; response to organic cyclic substance; response to nutrient; glycerol ether metabolic process

Research Articles on Trx2

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Product Notes

The Trx2 txn2 (Catalog #AAA3011811) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Trx2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Trx2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the Trx2 txn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Trx2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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