Rabbit anti-Human CREB3 Polyclonal Antibody | anti-CREB3 antibody

CREB3, NT (CREB3, LZIP, Cyclic AMP-responsive element-binding protein 3, Leucin zipper proitein, Luman, Transcription factor LZIP-alpha, Processed cyclic AMP-responsive element-binding protein 3) (MaxLight 550)

Cat. Num. AAA6288633

• Gene Names: CREB3; LZIP; LUMAN; sLZIP
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CREB3; Polyclonal Antibody; NT (CREB3; LZIP; Cyclic AMP-responsive element-binding protein 3; Leucin zipper proitein; Luman; Transcription factor LZIP-alpha; Processed cyclic AMP-responsive element-binding protein 3) (MaxLight 550); Processed cyclic AMP-responsive element-binding protein 3; anti-CREB3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CREB3.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-CREB3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 66-94 in the N-terminal region of human CREB3, UniProt Accession #O43889; NP_006359.3.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis in Jurkat cell line lysates (35ug/lane) usingMBS6012934. This demonstrates the CREB3 antibody detected the CREB3 protein (arrow).)

Related Product Information for anti-CREB3 antibody

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-response element and regulates cell proliferation. The protein interacts with host cell factor C1, which also associates with the herpes simplex virus (HSV) protein VP16 that induces transcription of HSV immediate-early genes. This protein and VP16 both bind to the same site on host cell factor C1. It is thought that the interaction between this protein and host cell factor C1 plays a role in the establishment of latency during HSV infection. This protein also plays a role in leukocyte migration, tumor suppression, and endoplasmic reticulum stress-associated protein degradation. Additional transcript variants have been identified, but their biological validity has not been determined.

Product Categories/Family for anti-CREB3 antibody

Antibodies; Transcription Factors

References

Kim, H.C., et al. Cell. Mol. Life Sci. 67(20):3499-3510(2010). Eleveld-Trancikova, D., et al. Mol. Immunol. 47 (11-12), 1963-1973 (2010): Kang, H., et al. Mol. Endocrinol. 23(11):1746-1757(2009). Mamdani, F., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (4), 500-504 (2008): Audas, T.E., et al. Mol. Cell. Biol. 28(12):3952-3966(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 38327637
• NCBI GeneID: 10488
• NCBI Accession #: NP_006359.3
• NCBI GenBank Nucleotide #: NM_006368
• UniProt Accession #: O43889; O14671; O14919; Q5TCV1; Q96GK8; Q9H2W3; Q9UE77; D0PTW6
• Molecular Weight: 39,580 Da
• NCBI Official Full Name: Homo sapiens cAMP responsive element binding protein 3 (CREB3), mRNA
• NCBI Official Synonym Full Names: cAMP responsive element binding protein 3
• NCBI Official Symbol: CREB3
• NCBI Official Synonym Symbols: LZIP; LUMAN; sLZIP
• NCBI Protein Information: cyclic AMP-responsive element-binding protein 3; CREB-3; basic leucine zipper protein; cAMP-responsive element-binding protein 3; cyclic AMP response element (CRE)-binding protein/activating transcription factor 1; leucin zipper proitein; small leucine zi
• UniProt Protein Name: Cyclic AMP-responsive element-binding protein 3
• Protein Family: CREB3 regulatory factor
• UniProt Gene Name: CREB3
• UniProt Synonym Gene Names: LZIP; CREB-3; cAMP-responsive element-binding protein 3; N-terminal Luman; Transcriptionally active form
• UniProt Entry Name: CREB3_HUMAN

NCBI Description

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-response element and regulates cell proliferation. The protein interacts with host cell factor C1, which also associates with the herpes simplex virus (HSV) protein VP16 that induces transcription of HSV immediate-early genes. This protein and VP16 both bind to the same site on host cell factor C1. It is thought that the interaction between this protein and host cell factor C1 plays a role in the establishment of latency during HSV infection. This protein also plays a role in leukocyte migration, tumor suppression, and endoplasmic reticulum stress-associated protein degradation. Additional transcript variants have been identified, but their biological validity has not been determined.[provided by RefSeq, Nov 2009]

Uniprot Description

CREB3: Endoplasmic reticulum (ER)-bound transcription factor that plays a role in the unfolded protein response (UPR). Involved in cell proliferation and migration, tumor suppression and inflammatory gene expression. Plays also a role in the human immunodeficiency virus type 1 (HIV-1) virus protein expression and in the herpes simplex virus-1 (HSV-1) latent infection and reactivation from latency. Isoform 2 plays a role in the unfolded protein response (UPR). Isoform 2 acts as a positive regulator of LKN-1/CCL15-induced chemotaxis signaling of leukocyte cell migration. Isoform 2 may play a role as a cellular tumor suppressor that is targeted by the hepatitis C virus (HSV) core protein. Isoform 2 represses the VP16-mediated transactivation of immediate early genes of the HSV-1 virus by sequestring host cell factor-1 HCFC1 in the ER membrane of sensory neurons, thereby preventing the initiation of the replicative cascade leading to latent infection. Isoform 3 functions as a negative transcriptional regulator in ligand-induced transcriptional activation of the glucocorticoid receptor NR3C1 by recruiting and activating histone deacetylases (HDAC1, HDAC2 and HDAC6). Isoform 3 decreases the acetylation level of histone H4. Isoform 3 does not promote the chemotactic activity of leukocyte cells. Belongs to the bZIP family. ATF subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: Golgi membrane; Golgi apparatus; nuclear body; endoplasmic reticulum membrane; membrane; cell soma; endoplasmic reticulum; cytoplasm; integral to membrane; integral to endoplasmic reticulum membrane; cytosol; nucleus

Molecular Function: protein dimerization activity; CCR1 chemokine receptor binding; protein binding; protein homodimerization activity; DNA binding; cAMP response element binding protein binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; cytoplasmic sequestering of transcription factor; viral reproduction; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of transcription of target genes involved in unfolded protein response; establishment of viral latency; chemotaxis; induction of positive chemotaxis; negative regulation of cell cycle; regulation of cell proliferation; reactivation of latent virus; positive regulation of transcription from RNA polymerase II promoter; regulation of cell growth; positive regulation of calcium ion transport; positive regulation of defense response to virus by host; positive regulation of cell migration