Western Blot (WB)
(Anti- TRPM8 Picoband antibody, MBS177667, Western blottingAll lanes: Anti TRPM8 (MBS177667) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: 22RV1 Whole Cell Lysate at 40ugLane 3: SW620 Whole Cell Lysate at 40ugLane 4: A549 Whole Cell Lysate at 40ugLane 5: A431 Whole Cell Lysate at 40ugPredicted bind size: 127KDObserved bind size: 127KD )
anti-Human TRPM8 Polyclonal Antibody | anti-TRPM8 antibody
Anti-TRPM8 Antibody
Western Blot (WB)
(Anti- TRPM8 Picoband antibody, MBS177667, Western blottingAll lanes: Anti TRPM8 (MBS177667) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: 22RV1 Whole Cell Lysate at 40ugLane 3: SW620 Whole Cell Lysate at 40ugLane 4: A549 Whole Cell Lysate at 40ugLane 5: A431 Whole Cell Lysate at 40ugPredicted bind size: 127KDObserved bind size: 127KD )
Western Blot (WB)
(Anti- TRPM8 Picoband antibody, MBS177667, Western blottingAll lanes: Anti TRPM8 (MBS177667) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: 22RV1 Whole Cell Lysate at 40ugLane 3: SW620 Whole Cell Lysate at 40ugLane 4: A549 Whole Cell Lysate at 40ugLane 5: A431 Whole Cell Lysate at 40ugPredicted bind size: 127KDObserved bind size: 127KD )
Background: Transient receptor potential cation channel subfamily M member 8 (TRPM8), also known as the cold and menthol receptor 1 (CMR1), is a protein that in humans is encoded by the TRPM8 gene. TRPM8 is an ion channel, upon activation it allows the entry of Na+ (sodium) and Ca2+ (calcium) ions to the cell that leads to depolarization and the generation of an action potential. The signal is conducted from primary afferents (type C- and A-delta) eventually leading to the sensation of cold and cold pain. The TRPM8 protein is expressed in sensory neurons, and it is activated by cold temperatures and cooling agents, such as menthol and icilin whereas WS-12 and CPS-369 are the most selective agonist of TRPM8.
NCBI and Uniprot Product Information
Uniprot Description
TRPM8: Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Channel, cation; Membrane protein, integral
Chromosomal Location of Human Ortholog: 2q37.1
Cellular Component: endoplasmic reticulum membrane; external side of plasma membrane; integral to membrane; lipid raft; plasma membrane
Molecular Function: calcium channel activity; protein binding; protein homodimerization activity
Biological Process: cellular calcium ion homeostasis; detection of temperature stimulus; protein homotetramerization; response to cold; thermoception
Research Articles on TRPM8
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Product Notes
The TRPM8 trpm8 (Catalog #AAA177667) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-TRPM8 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRPM8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the TRPM8 trpm8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRPM8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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