Rabbit anti-Human, Mouse TRPM5 Polyclonal Antibody | anti-TRPM5 antibody

TRPM5 Rabbit pAb

Cat. Num. AAA4758367

• Gene Names: TRPM5; MTR1; LTRPC5
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: TRPM5; Polyclonal Antibody; TRPM5 Rabbit pAb; transient receptor potential cation channel; subfamily M; member 5; MTR1; LTRPC5; anti-TRPM5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: -20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-TRPM5 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide of human TRPM5
• Conjugation: Unconjugated
• Modification: Unmodified
• Cell Position: Cell membrane, Membrane
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Mouse heart tissue, Primary antibody: 861187(TRPM5 Antibody) at dilution 1/1200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 861187(TRPM5 Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 861187(TRPM5 Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: ×200))

Product Categories/Family for anti-TRPM5 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 11225266
• NCBI GeneID: 29850
• NCBI Accession #: NP_055370
• NCBI GenBank Nucleotide #: NM_014555
• UniProt Accession #: Q9NZQ8
• Molecular Weight: 131kDa
• NCBI Official Full Name: transient receptor potential cation channel subfamily M member 5
• NCBI Official Synonym Full Names: transient receptor potential cation channel subfamily M member 5
• NCBI Official Symbol: TRPM5
• NCBI Official Synonym Symbols: MTR1; LTRPC5
• NCBI Protein Information: transient receptor potential cation channel subfamily M member 5
• UniProt Protein Name: Transient receptor potential cation channel subfamily M member 5
• Protein Family: Transient receptor potential cation channel subfamily
• UniProt Gene Name: TRPM5
• UniProt Synonym Gene Names: LTrpC-5; LTrpC5
• UniProt Entry Name: TRPM5_HUMAN

NCBI Description

This gene encodes a member of the transient receptor potential (TRP) protein family, which is a diverse group of proteins with structural features typical of ion channels. This protein plays an important role in taste transduction, and has characteristics of a calcium-activated, non-selective cation channel that carries Na+, K+, and Cs+ ions equally well, but not Ca(2+) ions. It is activated by lower concentrations of intracellular Ca(2+), and inhibited by higher concentrations. It is also a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. This gene is located within the Beckwith-Wiedemann syndrome critical region-1 on chromosome 11p15.5, and has been shown to be imprinted, with exclusive expression from the paternal allele. [provided by RefSeq, Oct 2010]

Uniprot Description

TRPM5: Voltage-modulated Ca(2+)-activated, monovalent cation channel (VCAM) that mediates a transient membrane depolarization and plays a central role in taste transduction. Monovalent- specific, non-selective cation channel that mediates the transport of Na(+), K(+) and Cs(+) ions equally well. Activated directly by increases in intracellular Ca(2+), but is impermeable to it. Gating is voltage-dependent and displays rapid activation and deactivation kinetics upon channel stimulation even during sustained elevations in Ca(2+). Also activated by a fast intracellular Ca(2+) increase in response to inositol 1,4,5- triphosphate-producing receptor agonists. The channel is blocked by extracellular acidification. External acidification has 2 effects, a fast reversible block of the current and a slower irreversible enhancement of current inactivation. Is a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. Heat activation is due to a shift of the voltage- dependent activation curve to negative potentials. Activated by arachidonic acid in vitro. May be involved in perception of bitter, sweet and umami tastes. May also be involved in sensing semiochemicals. Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM5 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cell soma; dendrite; integral to membrane; plasma membrane

Molecular Function: calcium activated cation channel activity; ion channel activity; potassium channel activity; sodium channel activity; voltage-gated ion channel activity

Biological Process: sensory perception of taste