Goat TRPC4 Polyclonal Antibody | anti-TRPC4 antibody

Goat Polyclonal to Human TRPC4

Cat. Num. AAA243706

• Gene Names: TRPC4; TRP4; HTRP4; HTRP-4
• Reactivity: Dog, Gorilla, Human, Rat; Predicted Reactivity: Gibbon, Mouse, Bovine, Hamster (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: TRPC4; Polyclonal Antibody; Goat Polyclonal to Human TRPC4; Anti-TRPC4 Antibody (C-Terminus) IHC-plus; CCE1; HTRP-4; HTRP4; TRP4; STRPC4; Trrp4; Trp-related protein 4; Human TRPC4; anti-TRPC4 antibody

• Host: Goat
• Reactivity: Dog, Gorilla, Human, Rat; Predicted Reactivity: Gibbon, Mouse, Bovine, Hamster (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human TRPC4. This antibody is expected to recognise all six reported isoforms (NP_057263.1; NP_003297.1; NP_001129427.1; NP_001129429.1; NP_001129428.1; NP_001129430.1).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 977

• Applicable Applications for anti-TRPC4 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (4.75 ug/ml), WB (0.3 - 1 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx. 110kD band observed in Rat Brain lysates (calculated MW of 112kD according to Human NP_057263.1 and to Rat NP_536321.1). Recommended concentration: 0.3-1 ug/ml.
• Target Species: Human
• Immunogen Description: Synthetic peptide C-KHAKEEDSSIDYD from an internal region (near C-terminus) of human TRPC4 (NP_057263.1; NP_003297.1; NP_001129427.1; NP_001129429.1; NP_001129428.1; NP_001129430.1). Percent identity by BLAST analysis: Human, Gorilla, Dog (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: TRPC4 antibody was raised against synthetic peptide C-KHAKEEDSSIDYD from an internal region (near C-terminus) of human TRPC4 (NP_057263.1; NP_003297.1; NP_001129427.1; NP_001129429.1; NP_001129428.1; NP_001129430.1). Percent identity by BLAST analysis: Human, Gorilla, Dog (100%); Gibbon, Mouse, Rat, Hamster, Bovine (92%); Marmoset, Elephant, Panda, Pig (85%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-TRPC4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 4.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-TRPC4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 4.75 ug/ml.)

Western Blot (WB)

(Antibody (0.3 ug/ml) staining of Rat Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-TRPC4 antibody

TRPC4 (or short transient receptor potential channel, TRP) contains 6 transmembrane domains, a pore region, a caveolin-binding domain and a PDZ binding domain at the C-terminus. It is a non-selective calcium permeant cation channel probably regulated by phosphatidylinositol second messengers via GPCRs or tyrosine kinases or depletion of internal calcium stores.

Product Categories/Family for anti-TRPC4 antibody

Family: Ion Channel; Subfamily: Calcium channel - TRP

NCBI and Uniprot Product Information

• NCBI GI #: 7706747
• NCBI GeneID: 7223
• NCBI Accession #: NP_057263.1
• NCBI GenBank Nucleotide #: NM_016179.2
• UniProt Accession #: Q9UBN4; Q15721; Q3SWS6; Q96P03; Q96P04; Q96P05; Q9UIB0; Q9UIB1; Q9UIB2; B1ALE0; B1ALE1; B1ALE2
• Molecular Weight: 36,759 Da
• NCBI Official Full Name: short transient receptor potential channel 4 isoform alpha
• NCBI Official Synonym Full Names: transient receptor potential cation channel, subfamily C, member 4
• NCBI Official Symbol: TRPC4
• NCBI Official Synonym Symbols: TRP4; HTRP4; HTRP-4
• NCBI Protein Information: short transient receptor potential channel 4; trp-related protein 4
• UniProt Protein Name: Short transient receptor potential channel 4
• Protein Family: Short transient receptor potential channel
• UniProt Gene Name: TRPC4
• UniProt Synonym Gene Names: TrpC4; hTrp-4; hTrp4
• UniProt Entry Name: TRPC4_HUMAN

NCBI Description

This gene encodes a member of the canonical subfamily of transient receptor potential cation channels. The encoded protein forms a non-selective calcium-permeable cation channel that is activated by Gq-coupled receptors and tyrosine kinases, and plays a role in multiple processes including endothelial permeability, vasodilation, neurotransmitter release and cell proliferation. Single nucleotide polymorphisms in this gene may be associated with generalized epilepsy with photosensitivity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

TRPC4: Form a receptor-activated non-selective calcium permeant cation channel. Acts as a cell-cell contact-dependent endothelial calcium entry channel. Probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Mediates cation entry, with an enhanced permeability to barium over calcium. May also be activated by intracellular calcium store depletion. Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Channel, calcium; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 13q13.3

Cellular Component: cortical cytoskeleton; cell surface; basolateral plasma membrane; plasma membrane; caveola; intercellular junction

Molecular Function: protein binding; store-operated calcium channel activity; cadherin binding; beta-catenin binding

Biological Process: axon guidance; calcium ion transport; gamma-aminobutyric acid secretion; oligodendrocyte differentiation; transmembrane transport