Rabbit anti-Human, Mouse PMCH Polyclonal Antibody | anti-PMCH antibody

PMCH, ID (PMCH, MCH, Pro-MCH, Neuropeptide-glycine-glutamic acid, Neuropeptide-glutamic acid-isoleucine, Melanin-concentrating hormone) (MaxLight 750)

Cat. Num. AAA6335156

• Gene Names: PMCH; MCH; ppMCH
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PMCH; Polyclonal Antibody; ID (PMCH; MCH; Pro-MCH; Neuropeptide-glycine-glutamic acid; Neuropeptide-glutamic acid-isoleucine; Melanin-concentrating hormone) (MaxLight 750); Melanin-concentrating hormone; anti-PMCH antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PMCH. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-PMCH antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 94-122 in the cenrtral terminal region of human PMCH.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (15ug/lane) usingMBS6008589.This demonstrates that MBS6008589 detected the PMCH protein (arrow).)

Western Blot (WB)

(Western Blot analysis in mouse brain tissue lysates (15ug/lane) usingMBS6008589.This demonstrates that MBS6008589 detected the PMCH protein (arrow).)

Western Blot (WB)

(Western Blot analysis of PMCH in 293 cell lysates (2ug/lane) usingMBS6008589. Lane 1: nontransfected Lane 2: transiently transfected with the PMCH gene.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue using MBS6008589 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6008589 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of K562 cells using MBS6008589 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PMCH antibody

Antibodies; Amino Acids

References

1. Kim, J.J., et al. J. Hum. Genet. 55(1):27-31(2010). 2. Gratacos, M., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 150B (6), 808-816 (2009). 3. Aziz, A., et al. Brain Pathol. 18(4):474-483(2008). 4. Kokkotou, E., et al. Proc. Natl. Acad. Sci. U.S.A. 105(30):10613-10618(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 71361684
• NCBI GeneID: 5367
• NCBI Accession #: NP_002665.2
• NCBI GenBank Nucleotide #: NM_002674.3
• UniProt Accession #: P20382; Q16044; Q8WVG0
• Molecular Weight: 18,679 Da
• NCBI Official Full Name: pro-MCH preproprotein
• NCBI Official Synonym Full Names: pro-melanin concentrating hormone
• NCBI Official Symbol: PMCH
• NCBI Official Synonym Symbols: MCH; ppMCH
• NCBI Protein Information: pro-MCH
• UniProt Protein Name: Pro-MCH
• Protein Family: Pro-MCH
• UniProt Gene Name: PMCH
• UniProt Synonym Gene Names: MCH; NGE; Neuropeptide G-E; NEI; Neuropeptide E-I; MCH
• UniProt Entry Name: MCH_HUMAN

NCBI Description

This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include melanin-concentrating hormone (MCH), neuropeptide-glutamic acid-isoleucine (NEI), and neuropeptide-glycine-glutamic acid (NGE). Melanin-concentrating hormone is a 19-amino acid neuropeptide that stimulates hunger and may additionally regulate energy homeostasis, reproductive function, and sleep. Pseudogenes of this gene have been identified on chromosome 5. [provided by RefSeq, Jul 2015]

Uniprot Description

PMCH: MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. May also have a role in spermatocyte differentiation. Belongs to the melanin-concentrating hormone family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q23-q24