Western Blot (WB)
(Western blot analysis of anti-AARS Pab in K562 cell line lysates (35ug/lane).AARS (arrow) was detected using the purified Pab.)
Rabbit anti-Human tRNA Synthetase, Alanyl Polyclonal Antibody | anti-AARS antibody
tRNA Synthetase, Alanyl, NT (AlaRS, Alanyl-tRNA Synthetase, Cytoplasmic, Alanine-tRNA Ligase, AlaRS, Renal Carcinoma Antigen NY-REN-42, AARS) (AP)
ELISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of anti-AARS Pab in K562 cell line lysates (35ug/lane).AARS (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-AARS Pab in K562 cell line lysates (35ug/lane).AARS (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AARS antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AARS antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
Uniprot Description
AARS: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. Defects in AARS are the cause of Charcot-Marie-Tooth disease type 2N (CMT2N). It is an axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies(designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. Belongs to the class-II aminoacyl-tRNA synthetase family.
Protein type: RNA-binding; Ligase; EC 6.1.1.7; Translation
Chromosomal Location of Human Ortholog: 16q22
Cellular Component: membrane; cytoplasm; cytosol
Molecular Function: amino acid binding; zinc ion binding; ATP binding; alanine-tRNA ligase activity; tRNA binding
Biological Process: protein folding; unfolded protein response; tRNA processing; regulation of translational fidelity; cerebellar Purkinje cell layer development; tRNA modification; response to amino acid stimulus; tRNA aminoacylation for protein translation; hair follicle development; gene expression; negative regulation of neuron apoptosis; neuromuscular process controlling balance; alanyl-tRNA aminoacylation
Disease: Epileptic Encephalopathy, Early Infantile, 29; Charcot-marie-tooth Disease, Axonal, Type 2n
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Product Notes
The AARS aars (Catalog #AAA6503697) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The tRNA Synthetase, Alanyl, NT (AlaRS, Alanyl-tRNA Synthetase, Cytoplasmic, Alanine-tRNA Ligase, AlaRS, Renal Carcinoma Antigen NY-REN-42, AARS) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's tRNA Synthetase, Alanyl can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-1:50 ELISA: 1:1,000 WB: 1:50-1:100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the AARS aars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "tRNA Synthetase, Alanyl, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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