Rabbit anti-Mouse ATG7 Polyclonal Antibody | anti-ATG7 antibody

RABBIT ANTI HUMAN ATG7 (C-TERMINAL)

Cat. Num. AAA223675

• Gene Names: Atg7; Agp7; Apg7l; Atg7l; Gm21553; 1810013K23Rik
• Reactivity: Mouse
• Applications: Immunohistochemistry, Western Blot
• Synonyms: ATG7; Polyclonal Antibody; RABBIT ANTI HUMAN ATG7 (C-TERMINAL); anti-ATG7 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 741

• Applicable Applications for anti-ATG7 antibody: Immunohistology Frozen, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 78kDa in Caco-2 cell lysate. ; Immunohistology - Frozen: Maximum Dilution: 10ug/ml; Western Blotting: Minimum Dilution: 1; Maximum Dilution: 2ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3)
• Immunogen: A peptide corresponding to an 17 amino acid sequence from near the carboxy terminus of human ATG7.; Buffer Solution
• Antiserum Preparation: Antiserum to human ATG7 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of whole cell lysates from Caco-2 human colorectal epitheloid adenocarcinoma cells probed with Rabbit anti Human ATG7 (MBS223675) at 0.5(A), 1(B) and 2(C) ug/ml)

Testing Data #2

(Immunocytochemical staining of MCF7 human breast adenocarcinoma cells with Rabbit anti Human ATG7 (MBS223675))

Related Product Information for anti-ATG7 antibody

Rabbit anti Human ATG7 (C-Terminal) antibody recognizes human Autophagy-related protein 7, known also as Ubitiquin Activating Enzyme-E1 like Protein, ATG7 and APG7, a 78kDa cytoplasmic protein. In mammalian cells, ATG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. Studies indicate that caspase-8 may alter ATG7 levels and thus the ATG7 program of autophagic cell death. ATG7 is evolutionally conserved and exhibits high homology within mammalian species. It expressed in wide range of tissues including kidney, liver, bone marrow and lymph nodes.

NCBI and Uniprot Product Information

• NCBI GI #: 358679369
• NCBI GeneID: 74244
• NCBI Accession #: NP_001240646.1
• NCBI GenBank Nucleotide #: NM_001253717.1
• Molecular Weight: 77,520 Da
• NCBI Official Full Name: ubiquitin-like modifier-activating enzyme ATG7 isoform 1
• NCBI Official Synonym Full Names: autophagy related 7
• NCBI Official Symbol: Atg7
• NCBI Official Synonym Symbols: Agp7; Apg7l; Atg7l; Gm21553; 1810013K23Rik
• NCBI Protein Information: ubiquitin-like modifier-activating enzyme ATG7; ATG12-activating enzyme E1 ATG7; autophagy-related protein 7; ubiquitin-activating enzyme E1-like protein
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme ATG7
• Protein Family: Ubiquitin-like modifier-activating enzyme
• UniProt Gene Name: Atg7
• UniProt Synonym Gene Names: Apg7l; APG7-like; mAGP7
• UniProt Entry Name: ATG7_MOUSE

Uniprot Description

ATG7: Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3. Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. Belongs to the ATG7 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Autophagy

Cellular Component: cytoplasm; axoneme; cytosol

Molecular Function: protein binding; protein homodimerization activity; small protein activating enzyme activity; APG8 activating enzyme activity; APG12 activating enzyme activity; transcription factor binding

Biological Process: positive regulation of apoptosis; regulation of protein ubiquitination; macroautophagy; pyramidal neuron development; adult walking behavior; post-embryonic development; protein transport; cardiac muscle cell development; transport; protein amino acid lipidation; protein catabolic process; neurite development; positive regulation of autophagy; autophagic vacuole formation; amino acid metabolic process; mitochondrion organization and biogenesis; central nervous system neuron axonogenesis; organelle organization and biogenesis; cerebellar Purkinje cell layer development; C-terminal protein lipidation; cellular response to starvation; liver development; neurological system process; cellular response to nitrogen starvation; response to starvation; protein modification by small protein conjugation; positive regulation of protein catabolic process; mitochondrion degradation; positive regulation of protein modification process; autophagy; cerebral cortex development; positive regulation of macroautophagy; negative regulation of apoptosis