Western Blot (WB)
(Western Blot analysis of CXorf34 expression in transfected 293T cell line by CXorf34 polyclonal antibody. Lane 1: CXorf34 transfected lysate (56.5kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human TRMT2B Polyclonal Antibody | anti-TRMT2B antibody
TRMT2B (tRNA (Uracil(54)-C(5))-methyltransferase Homolog, TRM2 Homolog, CXorf34, dJ341D10.3)
Gene Names
TRMT2B; CXorf34; dJ341D10.3
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
TRMT2B; Polyclonal Antibody; TRMT2B (tRNA (Uracil(54)-C(5))-methyltransferase Homolog; TRM2 Homolog; CXorf34; dJ341D10.3); Anti -TRMT2B (tRNA (Uracil(54)-C(5))-methyltransferase Homolog; anti-TRMT2B antibody
Host
Mouse
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human TRMT2B.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MAGLKRRVPLHSLRYFISMVGLFSKPGLLPWYARNPPGWSQLFLGTVCKGDFTRVIATKCQKGQKSQKKPSHLGPLDGSWQERLADVVTPLWRLSYEEQLKVKFAAQKKILQRLESYIQMLNGVSVTTAVPKSERLSCLLHPIIPSPVINGYRNKSTFSVNRGPDGNPKTVGFYLGTWRDGNVVCVQSNHLKNIPEKHSQVAQYYEVFLRQSPLEPCLVFHEGGYWRELTVRTNSQGHTMAIITFHPQKLSQEELHVQKEIVKEFFIRGPGAACGLTSLYFQESTMTRCSHQQSPYQLLFGEPYIFEELLSLKIRISPDAFFQINTAGAEMLYRTVGELTGVNSDTILLDICCGTGVIGLSLAQHTSRVLGIELLEQAVEDARWTAAFNGITNSEFHTGQAEKILPGLLKSKEDGQSIVAVVNPARAGLHYKVIQAIRNFRAIHTLVFVSCKLHGESTRNVIELCCPPDPAKKLLGEPFVLQQAVPVDLFPHTPHCELVLLFTR
Applicable Applications for anti-TRMT2B antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Immunogen
Full length human TRMT2B, aa1-504 (NP_079193.2).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of CXorf34 expression in transfected 293T cell line by CXorf34 polyclonal antibody. Lane 1: CXorf34 transfected lysate (56.5kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of CXorf34 expression in transfected 293T cell line by CXorf34 polyclonal antibody. Lane 1: CXorf34 transfected lysate (56.5kD). Lane 2: Non-transfected lysate.)
Product Categories/Family for anti-TRMT2B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
56,476 Da
NCBI Official Full Name
TRMT2B protein
NCBI Official Synonym Full Names
tRNA methyltransferase 2 homolog B (S. cerevisiae)
NCBI Official Symbol
TRMT2B
NCBI Official Synonym Symbols
CXorf34; dJ341D10.3
NCBI Protein Information
tRNA (uracil(54)-C(5))-methyltransferase homolog; tRNA (uracil(54)-C(5))-methyltransferase homolog; TRM2 homolog; TRM2 tRNA methyltransferase 2 homolog B; tRNA (uracil-5-)-methyltransferase homolog
UniProt Protein Name
tRNA (uracil(54)-C(5))-methyltransferase homolog
Protein Family
UniProt Gene Name
TRMT2B
UniProt Synonym Gene Names
CXorf34
UniProt Entry Name
TRM2_HUMAN
NCBI Description
This gene encodes a homolog of the TRM2 gene in S. cerevisiae. The yeast gene encodes a tRNA methyltransferase that plays a role in tRNA maturation. The yeast protein also has endo-exonuclease activity and may be involved in DNA double strand break repair. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Nov 2009]
Uniprot Description
TRMT2B: Probable S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the formation of 5-methyl-uridine at position 54 (m5U54) in all tRNA. May also have a role in tRNA stabilization or maturation. Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Methyltransferase; EC 2.1.1.35
Chromosomal Location of Human Ortholog: Xq22.1
Cellular Component: mitochondrion
Molecular Function: S-adenosylmethionine-dependent tRNA (m5U54) methyltransferase activity
Biological Process: RNA processing; tRNA processing; RNA methylation
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Product Notes
The TRMT2B trmt2b (Catalog #AAA6012784) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TRMT2B (tRNA (Uracil(54)-C(5))-methyltransferase Homolog, TRM2 Homolog, CXorf34, dJ341D10.3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRMT2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the TRMT2B trmt2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MAGLKRRVPL HSLRYFISMV GLFSKPGLLP WYARNPPGWS QLFLGTVCKG DFTRVIATKC QKGQKSQKKP SHLGPLDGSW QERLADVVTP LWRLSYEEQL KVKFAAQKKI LQRLESYIQM LNGVSVTTAV PKSERLSCLL HPIIPSPVIN GYRNKSTFSV NRGPDGNPKT VGFYLGTWRD GNVVCVQSNH LKNIPEKHSQ VAQYYEVFLR QSPLEPCLVF HEGGYWRELT VRTNSQGHTM AIITFHPQKL SQEELHVQKE IVKEFFIRGP GAACGLTSLY FQESTMTRCS HQQSPYQLLF GEPYIFEELL SLKIRISPDA FFQINTAGAE MLYRTVGELT GVNSDTILLD ICCGTGVIGL SLAQHTSRVL GIELLEQAVE DARWTAAFNG ITNSEFHTGQ AEKILPGLLK SKEDGQSIVA VVNPARAGLH YKVIQAIRNF RAIHTLVFVS CKLHGESTRN VIELCCPPDP AKKLLGEPFV LQQAVPVDLF PHTPHCELVL LFTR. It is sometimes possible for the material contained within the vial of "TRMT2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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