Western Blot (WB)
(Western blot analysis of TRMT1 expression in HL60 (A), mouse heart (B) whole cell lysates.)
Rabbit anti-Human, Mouse TRMT1 Polyclonal Antibody | anti-TRMT1 antibody
Anti-TRMT1 Antibody
Gene Names
TRMT1; TRM1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Purified by Immunogen Affinity Chromatography
Synonyms
TRMT1; Polyclonal Antibody; Anti-TRMT1 Antibody; tRNA (guanine(26)-N(2))-dimethyltransferase; tRNA 2 2-dimethylguanosine-26 methyltransferase; tRNA(guanine-26 N(2)-N(2)) methyltransferase; tRNA(m(2 2)G26)dimethyltransferase; anti-TRMT1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of TRMT1 protein.
Purity/Purification
Purified by Immunogen Affinity Chromatography
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Applicable Applications for anti-TRMT1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant fusion protein of human TRMT1. The exact sequence is proprietary.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of TRMT1 expression in HL60 (A), mouse heart (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of TRMT1 expression in HL60 (A), mouse heart (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of TRMT1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of TRMT1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-TRMT1 antibody
Rabbit polyclonal antibody to TRMT1
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
72,234 Da
NCBI Official Full Name
TRMT1 protein
NCBI Official Synonym Full Names
tRNA methyltransferase 1 homolog (S. cerevisiae)
NCBI Official Symbol
TRMT1
NCBI Official Synonym Symbols
TRM1
NCBI Protein Information
tRNA (guanine(26)-N(2))-dimethyltransferase; tRNA (guanine(26)-N(2))-dimethyltransferase; tRNA(m(2,2)G26)dimethyltransferase; TRM1 tRNA methyltransferase 1 homolog; tRNA(guanine-26,N(2)-N(2)) methyltransferase; tRNA 2,2-dimethylguanosine-26 methyltransfer
UniProt Protein Name
tRNA (guanine(26)-N(2))-dimethyltransferase
Protein Family
UniProt Gene Name
TRMT1
UniProt Entry Name
TRM1_HUMAN
Uniprot Description
Function: Dimethylates a single guanine residue at position 26 of most tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.
Catalytic activity: 2 S-adenosyl-L-methionine + guanine26 in tRNA = 2 S-adenosyl-L-homocysteine + N(2)-dimethylguanine26 in tRNA.
Sequence similarities: Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.Contains 1 C3H1-type zinc finger.Contains 1 Trm1 methyltransferase domain.
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Product Notes
The TRMT1 trmt1 (Catalog #AAA8308499) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TRMT1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TRMT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the TRMT1 trmt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRMT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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