Immunohistochemistry (IHC)
(Paraffin-embedded tissues of the hippocampal region (Fig. 1) and adrenal gland's medulla (Fig. 2) were prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:200 dilution of Cat. No. 06-574, Anti-TrkA Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity is expressed in human brain regions of higher cognitive processes and inside the adrenal medulla without nuclear involvement.)
Rabbit TrkA Polyclonal Antibody | anti-TrkA antibody
TrkA antibody
Reactivity
Reacts with Rat, Mouse and Human. Reactivity to other species is unknown. Recognizes TrKa, MW 140kDa. Does not crossreact with TrkB or TrkC.
Applications
Immunocytochemistry, Immunoprecipitation, Western Blot
Purity
TrkA antibody was purified by Protein A chromatography.
Synonyms
TrkA; Polyclonal Antibody; TrkA antibody; Polyclonal TrkA; Anti-TrkA; Trk-A; Trk A; anti-TrkA antibody
Host
Rabbit
Reactivity
Reacts with Rat, Mouse and Human. Reactivity to other species is unknown. Recognizes TrKa, MW 140kDa. Does not crossreact with TrkB or TrkC.
Clonality
Polyclonal
Isotype
IgG
Specificity
Rat
Purity/Purification
TrkA antibody was purified by Protein A chromatography.
Form/Format
Liquid, 100mM Tris-Glysine, 150mM Sodium Azide.
Concentration
1.0mg/ml (varies by lot)
Sequence Length
449
Applicable Applications for anti-TrkA antibody
Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Suitable for use in Immunoblot and Immunohistochemistry.
Biological Significance
TrkA, apparent molecular weight 140 kDa, is a high affinity nerve growth factor (NGF). The Trk proto-oncogene family contains four members, TrkA, Tr kb, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkA binds to nerve growth factor (NGF) and autophosphorylates on tyrosine residues (Tyr490, Tyr674, Tyr675, Tyr751 and Tyr785) to activate multiple downstream effector proteins.
Cross-Reactivity
Human, mouse
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling.
Immunogen
TrkA antibody was raised in rabbit using recombinant protein corresponding to extracellular domain of rat TrkA receptor as the immunogen.
Preparation and Storage
Aliquot and store at -20 degree C. Avoid repeated Freeze/Thaw cycles
Immunohistochemistry (IHC)
(Paraffin-embedded tissues of the hippocampal region (Fig. 1) and adrenal gland's medulla (Fig. 2) were prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:200 dilution of Cat. No. 06-574, Anti-TrkA Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity is expressed in human brain regions of higher cognitive processes and inside the adrenal medulla without nuclear involvement.)
Immunohistochemistry (IHC)
(Paraffin-embedded tissues of the hippocampal region (Fig. 1) and adrenal gland's medulla (Fig. 2) were prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:200 dilution of Cat. No. 06-574, Anti-TrkA Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity is expressed in human brain regions of higher cognitive processes and inside the adrenal medulla without nuclear involvement.)
Western Blot (WB)
(Western blot analysis of EGF-stimulated A431 cel lysate probed with anti-TrkA (2 ug/ml))
Western Blot (WB)
(Western blot analysis of EGF-stimulated A431 cel lysate probed with anti-TrkA (2 ug/ml))
Related Product Information for anti-TrkA antibody
Rabbit polyclonal TrkA antibody
Product Categories/Family for anti-TrkA antibody
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
140 kDa (predicted detection band MW)
NCBI Official Full Name
trkA
UniProt Protein Name
TrkA protein
Protein Family
UniProt Gene Name
trkA
UniProt Entry Name
G0L9U0_ZOBGA
Similar Products
Product Notes
The TrkA trka (Catalog #AAA535141) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TrkA antibody reacts with Reacts with Rat, Mouse and Human. Reactivity to other species is unknown. Recognizes TrKa, MW 140kDa. Does not crossreact with TrkB or TrkC. and may cross-react with other species as described in the data sheet. AAA Biotech's TrkA can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB). Suitable for use in Immunoblot and Immunohistochemistry. Researchers should empirically determine the suitability of the TrkA trka for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TrkA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
