Immunohistochemistry (IHC)
(Paraffin-embedded tissues of the hippocampal region (Fig. 1) and adrenal gland's medulla (Fig. 2) were prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Stained with MBS613956 (1:200). Immunoreactivity is expressed in human brain regions of higher cognitive processes and inside the adrenal medulla without nuclear involvement.)
Rabbit TrkA Polyclonal Antibody | anti-TrkA antibody
TrkA (Tyrosine Kinase Receptor A, Nerve Growth Factor Receptor, NGFR) (Biotin)
IHC-P: 1:200 detected TrkA in neurons of the hippocampal region and adrenal gland's medulla.
IP: 4ug immunoprecipitates TrkA from 500ug of PC-12 RIPA lysate.
Applications are based on unconjugated antibody.
WB: 0.5-2.0ug/ml detected TrkA in RIPA lysates from human A431 cells, TrkA in mouse 3T3 and rat PC-12 cell lysates. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with. Proteins were visualized using a Gt x Rb IgG (HRP) and a chemiluminescence detection system.
Immunohistochemistry (IHC)
(Paraffin-embedded tissues of the hippocampal region (Fig. 1) and adrenal gland's medulla (Fig. 2) were prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Stained with MBS613956 (1:200). Immunoreactivity is expressed in human brain regions of higher cognitive processes and inside the adrenal medulla without nuclear involvement.)
Immunohistochemistry (IHC)
(Paraffin-embedded tissues of the hippocampal region (Fig. 1) and adrenal gland's medulla (Fig. 2) were prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Stained with MBS613956 (1:200). Immunoreactivity is expressed in human brain regions of higher cognitive processes and inside the adrenal medulla without nuclear involvement.)
NCBI and Uniprot Product Information
NCBI Description
a protooncogene; receptor that binds to nerve growth factor (NGF) [RGD, Feb 2006]
Uniprot Description
TrkA: a receptor tyrosine kinase of the Trk family. High affinity receptor for nerve growth factor, neurotrophin-3 and neurotrophin-4/5 but not brain- derived neurotrophic factor. Known substrates include Shc, PI-3K, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system. Two splice variant isoforms have been described.
Protein type: Oncoprotein; Membrane protein, integral; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (receptor); EC 2.7.10.1; TK group; Trk family
Cellular Component: protein complex; cell surface; late endosome membrane; integral to plasma membrane; dendrite; early endosome; Golgi membrane; cell soma; early endosome membrane; axon; cytoplasm; late endosome; plasma membrane; cytoplasmic vesicle; receptor complex
Molecular Function: neurotrophin p75 receptor binding; protein binding; protein homodimerization activity; nerve growth factor receptor activity; ephrin receptor binding; protein-tyrosine kinase activity; nerve growth factor binding; transmembrane receptor protein tyrosine kinase activity; kinase binding; ATP binding
Biological Process: response to nicotine; circadian rhythm; axon guidance; mechanoreceptor differentiation; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; sensory perception of pain; olfactory nerve development; protein amino acid phosphorylation; positive regulation of synaptic transmission, glutamatergic; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; response to radiation; sympathetic nervous system development; learning and/or memory; response to axon injury; negative regulation of neuron apoptosis; detection of temperature stimulus involved in sensory perception of pain; response to electrical stimulus; response to hydrostatic pressure; aging; response to nutrient levels; response to drug; nervous system development; Sertoli cell development; detection of mechanical stimulus involved in sensory perception of pain; positive regulation of programmed cell death; positive regulation of angiogenesis; response to ethanol; B cell differentiation; positive regulation of Ras protein signal transduction; response to activity
Research Articles on TrkA
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Product Notes
The TrkA ntrk1 (Catalog #AAA6503754) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TrkA (Tyrosine Kinase Receptor A, Nerve Growth Factor Receptor, NGFR) (Biotin) reacts with Rat, Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TrkA can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP). ICC: 10ug/ml showed positive staining for TrkA in PC-12 cells fixed with 4% paraformaldehyde. IHC-P: 1:200 detected TrkA in neurons of the hippocampal region and adrenal gland's medulla. IP: 4ug immunoprecipitates TrkA from 500ug of PC-12 RIPA lysate. Applications are based on unconjugated antibody. WB: 0.5-2.0ug/ml detected TrkA in RIPA lysates from human A431 cells, TrkA in mouse 3T3 and rat PC-12 cell lysates. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with. Proteins were visualized using a Gt x Rb IgG (HRP) and a chemiluminescence detection system. Researchers should empirically determine the suitability of the TrkA ntrk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TrkA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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