Rabbit anti-Human, Mouse TRIM71 Polyclonal Antibody | anti-TRIM71 antibody

TRIM71 Antibody

Cat. Num. AAA9630244

• Gene Names: TRIM71; LIN41; LIN-41
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: TRIM71; Polyclonal Antibody; TRIM71 Antibody; 2610206G21Rik; Abnormal cell LINeage LIN 41; AL022943; E3 ubiquitin-protein ligase TRIM71; Gm1127; Homolog of C. elegans Lin 41; LIN 41; LIN41; LIN41_HUMAN; mlin 41; Protein lin 41 homolog; Protein lin-41 homolog; RGD1566388; Ripply2; TRIM 71; Tripartite motif containing 71; Tripartite motif-containing protein 71; anti-TRIM71 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: TRIM71 Antibody detects endogenous levels of TRIM71.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-TRIM71 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human TRIM71.
• Conjugation: Unconjugated
• Similarity: The NHL domain, containing the 6 NHL repeats, is necessary and sufficient to target RNA but not to repress mRNA. The minimal region needed to execute repression consists of the coiled coil domain and the Filamin repeat. The RING-type domain is dispensable for mRNA repression. Belongs to the TRIM/RBCC family.
• Post Translational Modifications: Autoubiquitinated.
• Subunit Structure: Interacts (via NHL repeats) with AGO2; the interaction increases in presence of RNA. Interacts with HSP90AA1. Interacts (via NHL repeats) with MOV10, PABPC1, PUM1, PUM2, STAU2, XRN1 and XRN2 in an RNA-dependent manner. Interacts with SHCBP1; leading to enhance its stability (By similarity).
• Tissue Specificity: Specifically expressed in testis.
• Function: E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance (Probable). Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A (By similarity). In addition, participates in post-transcriptional mRNA repression in a miRNA independent mechanism. Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1 (By similarity). Specific regulator of miRNA biogenesis. Binds to miRNA MIR29A hairpin and postranscriptionally modulates MIR29A levels, which indirectly regulates TET proteins expression.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-TRIM71 antibody

Cytoplasm; P-body; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 84993742
• NCBI GeneID: 131405
• NCBI Accession #: NP_001034200
• NCBI GenBank Nucleotide #: NM_001039111.1
• UniProt Accession #: Q2Q1W2
• Molecular Weight: 93,385 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase TRIM71
• NCBI Official Synonym Full Names: tripartite motif containing 71, E3 ubiquitin protein ligase
• NCBI Official Symbol: TRIM71
• NCBI Official Synonym Symbols: LIN41; LIN-41
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM71; protein lin-41 homolog; abnormal cell LINeage LIN-41; homolog of C. elegans Lin-41; tripartite motif-containing 71; tripartite motif-containing protein 71
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM71
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: TRIM71
• UniProt Synonym Gene Names: LIN41
• UniProt Entry Name: LIN41_HUMAN

Uniprot Description

TRIM71: May be involved in controlling the timing of organ formation during development. Belongs to the TRIM/RBCC family.

Chromosomal Location of Human Ortholog: 3p22.3

Molecular Function: miRNA binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: neural tube development; protein autoubiquitination; fibroblast growth factor receptor signaling pathway; neural tube closure; miRNA-mediated gene silencing, negative regulation of translation; G1/S transition of mitotic cell cycle